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Application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis

A terpene synthase, a technology for the diploid borer, the application in the prevention and control of the diploid borer C.suppressalis, the new application field of the tps46 gene in the rice insect resistance breeding, which can solve the problems such as identification difficulties and the like

Active Publication Date: 2019-03-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of volatile species in plants, it is very difficult to identify plant-specific terpene-based volatiles after insect pest induction

Method used

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  • Application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis
  • Application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis
  • Application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Cultivation and planting of T2 generation positive tps46 transgene overexpression rice:

[0034] For the cultivation of T0 generation TPS46 transgene overexpression-positive rice (Oe strains), please refer to our previous published article (Sun et al., 2017, Frontiers in plant science, 3:8-110), and more than 15 Oe strains were obtained. Among them, the Oe6, 7, 9 and 11 strains have the best overexpression effect, and are the main experimental materials of this application, and the Oe false positive strain is used as the OWt control. T0, T1 and T2 transgenic overexpressed tps46 rice seeds were first germinated in a light incubator with sterile water, and the catalytic conditions were as follows: temperature 22-28°C, photoperiod (day: night) 16h: 8h, relative humidity 50±5 %. Subsequently, each plant of the Oe6, Oe7, Oe9, Oe11 and OWt strains was planted in a plastic pot and placed in the greenhouse of Jiangsu Academy of Agricultural Sciences (24-32 ° C), and each st...

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Abstract

The invention discloses an application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis. The invention comprises tps46 transgenic overexpressed rice withobvious toxic effect on Chilo suppressalis larvae and rice secondary metabolite single product regulated by tps46 with obvious lethal effect. The compound having significant toxic effect on Chilo suppressalis larvae comprises methyl salicylate, ((E)-Beta-Farnesene, Linalool, (E)- Beta-Caryophyllene, alpha-humulene and limonene and mixtures thereof. The invention also comprises tps46 transgenic overexpressed rice and tps46-regulated rice secondary metabolite alpha-humulene and (E)-Beta-Farnesene, which can significantly attract Apanteles chilonis. Apanteles chilonis has a strong parasitic effect on Chilo suppressalis larvae, and can control Chilo suppressalis effectively and greenly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of the rice terpene synthase tps46 gene and its metabolites in preventing and treating C. suppressalis, and more specifically relates to the new application of the tps46 gene in rice insect-resistant breeding. Background technique [0002] In order to resist the harm of pests, plants have gradually formed a set of complex and effective defense mechanisms in the long-term co-evolution process with pests. After the pest damage is induced, the plant will enhance the release of specific volatile substances (Herbivore-Induced Plant Volatiles, HIPVs). On the one hand, these HIPVs can be used as a "help" signal for plants, recognized and utilized by natural enemies, and play a role in the indirect defense of plants. On the other hand, it can directly poison or repel pests. In recent years, the role of HIPVs in the three-level nutritional relationship of host plan...

Claims

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Application Information

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IPC IPC(8): A01N27/00A01N37/40A01N31/06A01N25/18A01P7/04A01P19/00
CPCA01N25/18A01N27/00A01N31/06A01N37/40
Inventor 孙洋刘宝生方继朝姚晓敏张永军杨磊张小娜
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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