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Application of rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers

A terpene synthase and rice technology, applied in the biological field, can solve problems such as limiting rice production potential, accelerating drug resistance, and difficult control, and achieve the effect of trapping and comprehensive control and reducing harm

Active Publication Date: 2018-11-23
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the harm of borers such as Chilo suppressalis Walker and Sesamia inferens Walker has been the main pest problem in rice production, seriously affecting the high-quality, high-yield and stable yield of rice, and severely limiting the potential of rice production.
At present, chemical control is the main control method for borers in the field, but the intensive use of insecticides has accelerated the emergence of drug resistance, and has aroused strong public concerns about food security and environmental pollution.
In addition, borer eggs bore into rice stems shortly after hatching, and complete their entire larval and pupal stages in the rice stalks, making it very difficult to control them with conventional biological or chemical insecticides

Method used

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  • Application of rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers
  • Application of rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers
  • Application of rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers

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Embodiment 1

[0028] 1. Cultivation and planting of T2 generation positive TPS46 transgene overexpression rice:

[0029]For the cultivation of T0 generation TPS46 transgene overexpression-positive rice (Oe strains), please refer to our previous published article (Sun et al., 2017, Frontiers in plant science, 3:8-110), and more than 15 Oe strains were obtained. Among them, the Oe6, 7, 9 and 11 strains have the best overexpression effect, and are the main experimental materials of this application, and the Oe false positive strain is used as the OWt control. T0, T1 and T2 generations of transgenic overexpressed TPS46 rice seeds were first germinated in a light incubator with sterile water, and the catalytic conditions were as follows: temperature 22-28 ° C, photoperiod (day: night) 16h: 8h, relative humidity 50±5 %. Subsequently, each plant of the Oe6, Oe7, Oe9, Oe11 and OWt strains was planted in a plastic pot and placed in the greenhouse of the Jiangsu Academy of Agricultural Sciences (24-...

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Abstract

The invention discloses application of a rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers and also includes a pink rice borer attractant. The pink rice borer attractant contains the rice terpene synthetase TPS46 gene or transgenic rice thereof after overexpression. The pink rice borer attractant also contains a mixture of (E)-beta-farnesene, methylsalicylic acid, (E)-alpha-bergamotene, (E)-beta-caryophyllene, alpha-humuleno, linalool, alpha-bisabolene and limonene. The rice TPS46 gene which makes overexpression on rice plants and can remarkably lure spontaneousgeneration of the pink rice borers is authenticated for the first time; and field tests show that for rice with overexpression of the TPS46 gene, the harms of the pink rice borers to non-transgenic control rice can be remarkably lowered, and field attraction and comprehensive prevention and control of the pink rice borers are facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of the rice terpene synthase TPS46 gene in the prevention and treatment of the large borer, and more specifically relates to the new application of the TPS46 gene transgenic overexpression crop and its regulation mixture. Background technique [0002] During the long-term co-evolution process, in order to resist the feeding hazards of pests, plants have gradually formed a complex and effective defense system. After the pests are infested, the plants will enhance the release of specific pest-induced volatiles (Herbivore-Induced Plant Volatiles, HIPVs). "Signals, recognized and utilized by natural enemies, play an important role in indirect defenses of plants. In recent years, the role of HIPVs in the three-level nutritional relationship between host plant-pest-natural enemy and its molecular regulation mechanism, as well as the exploration of its applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01N37/38A01N31/02A01G13/00A01P7/04A01P19/00
CPCA01G13/00A01N27/00A01N31/02A01N37/38
Inventor 孙洋刘宝生方继朝姚晓敏张永军
Owner JIANGSU ACAD OF AGRI SCI
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