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Method for preparing beta-farnesene by fermentation of sugar sorghum stalks and sugarcane waste residues

A technology for sugarcane waste bagasse and sorghum stalk, which is applied in the field of preparing beta-farnesene, can solve the problems of high cost and high cost of beta-farnesene, and achieve the effects of reduced preparation cost, low cost and easy availability of raw materials

Active Publication Date: 2016-10-12
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with Brazil, the cost of sugar cane in China is higher, mainly for food or sugar production, and the cost of producing β-farnesene is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing β-farnesene by fermenting sugar sorghum stalks and sugarcane waste residue, using the following steps:

[0022] A: Harvest the mature sugar sorghum from the sugar sorghum planting field, remove the sorghum spikes, roots and leaves, then wash and remove the dust and soil on the sugar sorghum stalks, and press them into a press to obtain sugar sorghum juice;

[0023] B: filtering, evaporating, and concentrating the sugar sorghum juice obtained in step A to obtain a sugar sorghum juice concentrate with a water content of 20%;

[0024] C: mix the sugar sorghum juice concentrate obtained in step B with the by-product sugarcane waste residue produced by the sugar factory in a mass ratio of 1:1;

[0025] D: Weigh an appropriate amount of the mixture of sugar sorghum and sugarcane waste residue obtained in step C as a carbon source for fermentation, use Saccharomyces cerevisiae with a preservation number of CICC No. 32475 as the strain, and culture at a s...

Embodiment 2

[0031] A method for preparing β-farnesene by fermenting sugar sorghum stalks and sugarcane waste residue, using the following steps:

[0032] A: Harvest the mature sugar sorghum from the sugar sorghum planting field, remove the sorghum spikes, roots and leaves, then wash and remove the dust and soil on the sugar sorghum stalks, and press them into a press to obtain sugar sorghum juice;

[0033] B: filtering, evaporating, and concentrating the sugar sorghum juice obtained in step A to obtain a sugar sorghum juice concentrate with a water content of 22%;

[0034] C: mix the sugar sorghum juice concentrate obtained in step B with the by-product sugarcane waste residue produced by the sugar factory in a mass ratio of 2:1;

[0035] D: Weigh an appropriate amount of the mixed solution of sugar sorghum and sugarcane waste residue obtained in step C as the carbon source for fermentation, use Saccharomyces cerevisiae with the preservation number CICC No. 32475 as the strain, and cultur...

Embodiment 3

[0041] A method for preparing β-farnesene by fermenting sugar sorghum stalks and sugarcane waste residue, using the following steps:

[0042] A: Harvest the mature sugar sorghum from the sugar sorghum planting field, remove the sorghum spikes, roots and leaves, then wash and remove the dust and soil on the sugar sorghum stalks, and put them into a press for pressing to obtain sugar sorghum juice;

[0043] B: filtering, evaporating, and concentrating the sugar sorghum juice obtained in step A to obtain a sugar sorghum juice concentrate with a water content of 23%;

[0044] C: mix the sugar sorghum juice concentrate obtained in step B with the by-product sugarcane waste residue produced by the sugar factory in a mass ratio of 3:1;

[0045] D: Weigh an appropriate amount of the mixed solution of sugar sorghum and sugarcane waste residue obtained in step C as the carbon source for fermentation, use Saccharomyces cerevisiae with the preservation number of CICC No. 32475 as the stra...

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Abstract

The invention relates to a method for preparing beta-farnesene by fermentation of sugar sorghum stalks and sugarcane waste residues. The method comprises the following steps of: A, harvesting and squeezing mature and fresh sugar sorghum to obtain sugar sorghum juice; B, concentrating the sugar sorghum juice; C, uniformly mixing a sugar sorghum juice concentrated solution with the sugarcane waste residues which are by-products produced in a sugar refinery, and obtaining a mixed solution of the sugar sorghum and the sugarcane waste residues; D, taking the mixed solution of the sugar sorghum and the sugarcane waste residues as a carbon source of fermentation, and taking Saccharomyces cerevisiae of which the collection number is CICC No. 32475 as a strain to prepare a shake flask strain; E, inoculating the shake flask strain to a seed culture medium to be cultured; G, inoculating a seed culture fluid to a fermentation medium to be fermented for 30 to 50 hours, adding a flocculating agent to a fermentation broth, and obtaining a supernatant through siphoning after precipitation. According to the method for preparing beta-farnesene by the fermentation of the sugar sorghum stalks and the sugarcane waste residues disclosed by the invention, the adopted raw materials are low in cost, and the content of the beta-farnesene in the obtained supernatant is high, thereby being beneficial to reduction of the preparation cost of squalane derived from plants.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing β-farnesene by fermenting sugar sorghum stalks and sugarcane waste residue. Background technique [0002] β-Farnesene, also known as farnesene, is a linear olefin with 15 carbons. β-Farnesene can be used as the precursor of diesel oil and special chemicals, and also the precursor of important fragrance components. The products after further synthesis can be used in cosmetics, flavors and fragrances, polymers, lubricants, diesel, jet fuel and other fields. At present, β-farnesene is mainly prepared by dehydration of nerolidol under the action of various acidic catalysts. The existence of many isomers leads to complicated separation process and high production cost. [0003] The development of biotechnology provides a low-cost, renewable and sustainable way for the directional preparation of high-purity β-farnesene. Amyris of the United State...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/865
CPCC12P5/026
Inventor 岳娟毕天宇刘丽仙邱玉想毕永贤蒋丽刚
Owner PROYA COSMETICS
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