Compound mutagenesis breeding method of mutant strain for highly yielding beta-farnesene

Abstract

The invention relates to the technical field of biological mutagenesis breeding, and in particular, relates to a compound mutagenesis breeding method of a mutant strain for highly yielding beta-farnesene. The method includes the steps: preparation of a bacterial suspension, plasma-ultraviolet compound mutagenesis, flat plate primary screening, shake flask re-screening and fermentation tank verification. According to the compound mutation breeding method of the mutant strain for highly yielding beta-farnesene, through plasma-ultraviolet compound mutagenesis, flat plate primary screening and shake flask re-screening are adopted, and finally, the fermentation unit of beta-farnesene of the mutant strain for highly yielding beta-farnesene in the fermentation tank is 169.10% higher than that ofa starting strain, and the mutant strain still has good hereditary stability through 8 times of consecutive passage.

Description

technical field

[0001] The invention relates to the technical field of biological mutation breeding, in particular to a compound mutation breeding method for high-yielding β-farnesene mutant strains. Background technique

[0002] β-Farnesene (also known as β-Farnesene) is a chain sesquiterpene compound composed of three isoprenes, which is widely used in daily chemical, agricultural, chemical, energy and pharmaceutical industries. The field has wide application. Especially in the field of medicine, β-farnesene is an important raw material for the synthesis of isophytic alcohol, the precursor of vitamin E. Vitamin E is one of the vitamin products with the largest market capacity in the world, with an annual global production of about 60,000 tons. The raw material supply of vitamin E precursor isophytic alcohol has been monopolized by BASF in Germany and DSM in the Netherlands. They use citral as an intermediate Synthesize isophytol in vivo, and then use the total chemical s...