SiRNA based on Sitobion avenae SaveOrco gene design and application thereof

A technology of tube aphid and gene, applied in the direction of DNA / RNA fragment, application, genetic engineering, etc., can solve the problem of reducing the control effect

Active Publication Date: 2016-07-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the effective implementation of the above measures depends on the cooperation of environmental conditions, wind, rain, low temperature, etc. will reduce the control effect

Method used

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  • SiRNA based on Sitobion avenae SaveOrco gene design and application thereof
  • SiRNA based on Sitobion avenae SaveOrco gene design and application thereof
  • SiRNA based on Sitobion avenae SaveOrco gene design and application thereof

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Embodiment Construction

[0021] Below in conjunction with example the present invention is described in further detail.

[0022] 1. Materials and methods

[0023] 1.1 Insects to be tested

[0024] The experimental population of the monosexual line of Aphid sativa was originally derived from the wild population in Langfang, multiplied by a single head, and fed to wheat plants. The wheat variety is Zhongmai 175. Select low-density (10 wheat seedlings / pot, average 1 head / plant) to feed more than 3 generations of Aphid aphid adults and their offspring as test insects. The breeding temperature was maintained at 22±1°C, the relative humidity was 75%, and the photoperiod was 16h︰8h.

[0025] 1.2 siRNA sequence design

[0026] Using the cDNA of SaveOrco (Genbank accession number: GQ275379) as a template, siRNA was designed and synthesized according to the principle of siRNA sequence design, named SiOrco7, and siRNA with scrambled sequence was used as a negative control, named NegOrco7. See Table 1 for seq...

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Abstract

The invention relates to siRNA based on Sitobion avenae SaveOrco gene design and application thereof.Transcription of a SaveOrco gene is interfered through feeding of siOrco7.Real-time fluorescent quantitative polymerase chain reaction (PCR) research shows that the expression of the SaveOrco gene is obviously restrained.Olfactory behavior reaction detection results show that the responding rates of silenced aphids to z-3-hexenol, methyl salicylate and EBF are obviously reduced under SaveOroco expression.An EBF inducing experiment for sitobion avenae winged morph differentiation is developed, siRNA processed aphids stop inducing of EBF, normal chemical communication of the aphids is interfered, and stress inducible wing bud generation is also stopped.In this way, a conserved sequence of the Orco gene can be applied to an RNAi technology mediated by siRNA, and the aphids can be effectively restrained from rapidly diffusing to be harmful and are prevented and treated.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the siRNA sequence of the olfactory receptor gene SaveOrco of the long tube aphid Sitobionavenae and its application in the field of aphid prevention and control. Background technique [0002] Aphids have a wide variety of species and a wide range of hosts, many of which are important agricultural pests. It feeds on thorns and feeds on the phloem juice of host plants, and causes serious losses in the yield and quality of host crops by plundering host nutrients, carrying and spreading plant viruses, and excreting honeydew. Insects mainly use their sense of smell to recognize their surrounding habitats, and aphids are no exception, such as the location of host plants at close range [WebsterBEN (2012) Theroleofolfactioninaphidhostlocation.PhysiologicalEntomology37:10-18.] or the recognition of pheromones [MustapartaH (1990) Chemicalinformationprocessingintheolfactorysystemofinsect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A01N57/16A01P7/04
CPCA01N57/16C12N15/1138C12N2310/14
Inventor 范佳陈巨莲蒋欣张勇孙京瑞
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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