Stress inducible gene promoter and application thereof

A promoter and nucleic acid molecule technology, applied in the fields of plant bioengineering and plant improvement genetic engineering, can solve problems such as long waiting time, target gene expression time (developmental stage-specific space improvement effect is not obvious, plant growth and development impact, etc.)

Inactive Publication Date: 2011-09-14
BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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Problems solved by technology

At present, some strong constitutive promoters are widely used in the field of agricultural biotechnology, such as the CaMV 35S promoter and the maize Ubiquitin-1 promoter (Battraw and Hall, 1990; Christensen et al. 1992), however, in the use of these promoters When transforming rice and other crops by sub-inducing the target gene to improve the quality of the crop, the improvement effect is not obvious because the time (developmental stage specificity) or space (tissue and organ specificity) of the expression of the target gene cannot be well controlled, or Because these constitutive promoters induce too high gene expression and affect the growth and development of plants, these are the obstacles encountered when using constitutive strong promoters combined with functional genes

Method used

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  • Stress inducible gene promoter and application thereof
  • Stress inducible gene promoter and application thereof
  • Stress inducible gene promoter and application thereof

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Embodiment Construction

[0026] The methods used in the following examples are conventional methods unless otherwise specified. The primers used were synthesized by Shanghai Yingjun Biotechnology Co., Ltd., and the sequencing was completed by Beijing Huada Gene. Zibao Bioengineering Co., Ltd., pEASY-T1 ligation kit was purchased from Beijing Quanshijin Biotechnology Co., Ltd., and T4 DNA ligase was purchased from Promega Company. The methods were all carried out according to the methods provided in the kit. The carrier pHPG used in the experiment was transformed from this experiment, and the basic skeleton came from pCAMBIA1303 of CAMBIA Company.

[0027] 1. Stress expression profile analysis of KT584 gene

[0028] The rice Zhonghua 11 material at the two-leaf stage was selected for stress treatment. The materials and samples of the drought treatment were collected in the following stages: period 1 (D1): slightly rolled leaves, relative water content (RWC) at 90%-95%; period 2 (D2): half-rolled leav...

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Abstract

The invention discloses a method for regulating expression of a heterologous nucleotide sequence in plants and a DNA carrier. The DNA carrier comprises the nucleotide sequence of a new plant stress inducible gene promoter. The invention provides the method for expressing the heterologous nucleotide sequence in plants by using the sequence of the stress inducible gene promoter disclosed by the invention.

Description

technical field [0001] The invention belongs to the technical fields of plant bioengineering and plant improvement genetic engineering, and specifically relates to the isolation, identification and application of a plant stress-induced expression promoter. Background technique [0002] Plant stress refers to environmental factors that adversely affect the normal growth and development of plants. Studies have found that the stresses that have important impacts on plants mainly include abiotic stresses such as drought, low temperature, salinity, and high temperature, and biotic stresses such as pathogens. Both abiotic and biotic stresses will cause a decline in the yield and quality of various crops. Therefore, cultivating and promoting stress-resistant varieties is an effective way to ensure stable and high yields of crops. Using the plant's own stress-resistant genes, new stress-resistant varieties can be bred through conventional breeding and molecular marker-assisted bree...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/82C12N15/113C12N1/21C12N1/15
Inventor 李早霞李晓娟刘敏夏勉周君莉
Owner BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED
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