Stress inducible gene promoter and application thereof

Abstract

The invention discloses a method for regulating expression of a heterologous nucleotide sequence in plants and a DNA carrier. The DNA carrier comprises the nucleotide sequence of a new plant stress inducible gene promoter. The invention provides the method for expressing the heterologous nucleotide sequence in plants by using the sequence of the stress inducible gene promoter disclosed by the invention.

Description

technical field [0001] The invention belongs to the technical fields of plant bioengineering and plant improvement genetic engineering, and specifically relates to the isolation, identification and application of a plant stress-induced expression promoter. Background technique [0002] Plant stress refers to environmental factors that adversely affect the normal growth and development of plants. Studies have found that the stresses that have important impacts on plants mainly include abiotic stresses such as drought, low temperature, salinity, and high temperature, and biotic stresses such as pathogens. Both abiotic and biotic stresses will cause a decline in the yield and quality of various crops. Therefore, cultivating and promoting stress-resistant varieties is an effective way to ensure stable and high yields of crops. Using the plant's own stress-resistant genes, new stress-resistant varieties can be bred through conventional breeding and molecular marker-assisted bree...

AI Technical Summary

Problems solved by technology

At present, some strong constitutive promoters are widely used in the field of agricultural biotechnology, such as the CaMV 35S promoter and the maize Ubiquitin-1 promoter (Battraw and Hall, 1990; Christensen et al. 1992), however, in the use of these promoters When transforming rice and other crops by sub-inducing the target gene to improve the quality of the crop, the improvement effect is not obvious because the time (developmental stage specificity) or space (tissue and organ specificity) of the expression of the target gene cannot be well controlled, or Because these constitutive promoters induce too high gene expression and affect the growth and development of plants, these are the obstacles encountered when using constitutive strong promoters combined with functional genes to improve crop quality.

[0005] In addition, when studying certain metabolic processes or regulatory pathways, it is often necessary to transform two or more genes on the same pathway into the same line, transforming one of the genes to obtain transgenic plants and then transforming another gene, or It takes a long time to wait for the hybridization after the two genes are transformed separately. In order to improve the efficiency and shorten the time for multiple gene transformation, it has recently been reported that a new vector can be used to simultaneously transform multiple genes (Lin et al. 2003; Chen et al. 2006), but if the same promoter is used repeatedly during multigene transformation, it may also lead to gene silencing due to the high homology of the promoter sequence

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