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Diagnostic systems and method for determining the presence of a genotoxic and/or toxic compound in a sample

Inactive Publication Date: 2002-10-29
VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK NV VITO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

A last aim of the present invention is to provide such a diagnostic system and method which allow an automatic screening upon very small volume of sample.

Problems solved by technology

However, some compounds act directly on the light production (e.g. aldehydes, organic solvents) or enhance the metabolism of the bacteria creating false-positive results.

Method used

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  • Diagnostic systems and method for determining the presence of a genotoxic and/or toxic compound in a sample
  • Diagnostic systems and method for determining the presence of a genotoxic and/or toxic compound in a sample
  • Diagnostic systems and method for determining the presence of a genotoxic and/or toxic compound in a sample

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Experimental program
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Embodiment Construction

The present invention is related to a diagnostic system made of

a transformed micro-organism capable of an increased reporter activity upon exposure to an environmental insult, preferably exposure to a genotoxic compound, said micro-organism having a stress inducible promoter sequence, preferably a promoter sequence which is inducible by a genotoxic compound, said promoter sequence being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed, and of

a transformed micro-organism having a constitutive and non-stress inducible promoter sequence, preferably a constitutive promoter sequence which is not inducible by said genotoxic compound, said promoter sequence being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed.

Preferably, both transformed micro-organisms are bioluminescent micro-organisms and the signal can be assayed a...

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Abstract

This invention concerns a diagnostic system made of: a transformed microorganism capable of an increased reporter activity upon exposure to an environmental insult, said microorganism having a stress inducible promoter sequence being operatively linked to a reported encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed, and of a transformed microorganism having a constitutive and non stress inducible promoter sequence being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed.

Description

The present invention is related to a diagnostic system and to a method for determining the presence of a genotoxic compound in a sample.BACKGROUND OF THE INVENTION AND STATE OF THE ARTThe International Patent Application PCT / EP96 / 01745 describes a recombinant nucleic acid sequence, a host micro-organism comprising said nucleic acid sequence and the use of said host micro-organism for detecting the presence of genotoxic compounds in a sample. Said bacterial genotoxicity test is based on bioluminescence and allows an easy, very rapid and low cost detection of genotoxic compounds. The test was shown to be at least as sensitive as the Ames test and SOS-chromotest and to allow genotoxicity kinetics measurements as well as a simultaneous evaluation of the toxicity of the test compound or -material (van der Lelie et al., 1997). This new test, referred to as the VITOTOX.RTM. test was therefore considered to be a valuable short-term genotoxicity and toxicity test for many different purposes...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/02G01N33/50C12N1/21C12N15/09C12Q1/66C12Q1/6897C12R1/19G01N33/15
CPCC12Q1/02C12Q1/6897
Inventor VAN DER LELIE, DANIELREGNIERS, LUC AGNES LOUIS JEAN BOSCOTAGHAVI, SAFIYHCORBISIER, PHILIPPE GILBERT GHISLAINVERSCHAEVE, LUC PHILIPPINE EDOUARD
Owner VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK NV VITO
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