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Application of stress inducible promoter on controlling gene in drought induced expression

A drought-induced and induced expression technology, applied in the fields of application, angiosperms/flowering plants, DNA/RNA fragments, etc.

Inactive Publication Date: 2012-07-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of whether the OsIP3KP promoter in rice can be induced by abiotic stress (drought, high salinity, ABA) to specifically increase the expression of downstream target genes, there is no relevant report

Method used

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  • Application of stress inducible promoter on controlling gene in drought induced expression
  • Application of stress inducible promoter on controlling gene in drought induced expression
  • Application of stress inducible promoter on controlling gene in drought induced expression

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Experimental program
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Effect test

Embodiment 1

[0020] 1. Isolation of the promoter of the OsIP3K gene

[0021] Real-time PCR is used to detect that OsIP3KP is induced by drought, high salt, and abscisic acid (ABA). The induction of OsIP3KP is regulated by an upstream promoter sequence. From the rice genome annotation database (promoter downstream gene retrieval address of the present invention: http: / / rice.plantbiology.msu.edu / cgi-bin / ORF_infopage.cgi,) can be found in the gene, the accession number in the mutant database of the above website is LOC_Os03g12840.1, and the upstream of the promoter is the promoter of the gene sequence.

[0022] According to the sequence comparison of the OsIP3K gene sequence in the NCBI (http: / / www.ncbi.nlm.nih.gov / ) database of the National Center for Biotechnology Information in the United States, the match of the OsIP3K gene on the whole genome of rice that has been sequenced and submitted was found Position, the position of the transcription start site ATG on the genome can be determined...

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Abstract

The invention belongs to the field of plant gene engineering and particularly relates to separation, clone and an abiotic stress (drought, high salt and abscisic acid ABA) inducible prompter OsIP3KP obtained through functional verification. The promoter has a nucleotide sequence expressed by SEQ ID NO: 1 and has a sequence length of 2119 bp. Through the promoter, the specific expression of an anti-stress related gene in the abiotic stress environment can be improved, and the promoter is applied to the genetic improvement on the abiotic stress resisting capacity of rice. According to the invention, the rice-controlling abiotic stress inducible promoter OsIP3KP is cloned by adopting a PCR (Polymerase Chain Reaction) amplification and fusion report gene genetic transformation method; and through realtime-PCR, reporter gene GUS (Glucuronidase) staining observation and enzyme activity assay, the cloned prompter is proved to be induced by the abiotic stress (drought, high salt and abscisic acid ABA) and can be applied to the genetic improvement on rice and other plants.

Description

technical field [0001] The invention relates to the field of rice genetic engineering. It specifically involves the isolation, cloning and functional verification of a promoter induced by abiotic stress (drought, high salinity, ABA), which can improve the specific expression of stress-resistance-related genes in an abiotic stress environment, and is applied to genetic improvement of rice resistance to abiotic stress. capacity for biological adversity. The present invention adopts the method of PCR amplification and fusion reporter gene genetic transformation, and clones the rice abiotic stress-induced promoter OsIP3KP to control it. Through real time-PCR, reporter gene GUS staining observation and enzyme activity measurement, it is proved that the promoter is influenced by abiotic stress. Adversity (drought, high salinity, ABA) induction can be applied to the genetic improvement of rice. Background technique [0002] In addition to its inherent genetic basis, plant growth ...

Claims

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Application Information

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IPC IPC(8): C12N15/113A01H5/00
Inventor 熊立仲都浩
Owner HUAZHONG AGRI UNIV
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