PCR-RFLP method for detecting single nucleotide polymorphism of gene CREB 1

A technique of PCR-RFLP and single nucleotide polymorphism, which is applied in the fields of biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve the problems that the correlation of CREB1 gene type II diabetes has not been reported yet, and is easy to be popularized and applied , low cost, high precision effect
CN110734969AInactive Publication Date: 2020-01-31WUHAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV OF SCI & TECH
Publication Date
2020-01-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a PCR-RFLP method for detecting the single nucleotide polymorphism of a gene CREB 1. Firstly, according to a DNA pool sequencing result, the detected gene polymorphism comprises single nucleotide polymorphism of T or G at the 1354th site from a CREB1 gene promoter region to a transcription starting point and single nucleotide polymorphism of T or A at the 1343th site. PCR amplification is conducted on a CREB1 gene sequence containing polymorphic sites in the presence of Taq DNA polymerase, Buffer (a buffer environment), Mg++ and dNTPs by taking genome DNA of a to-be-detected sample as a template and a primer pair P as a primer, a PCR product is divided into two parts, the two parts are digested by using restriction enzymes Hha I and Xsp I respectively, and enzyme digestion products are subjected to typing through agarose gel electrophoresis.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology detection, and in particular relates to a PCR-RFLP method for detecting single nucleotide polymorphism of gene CREB1. Background technique

[0002] Diabetes is considered to be the fifth most serious disease that endangers human health in the world, and 90% of them are non-insulin-dependent diabetes, also known as type II diabetes. The disease is a complex metabolic disorder, and its pathogenesis is complex, which is the result of the interaction between genetics and the environment, among which genetic polymorphism may be the main factor affecting the susceptibility of the disease among individuals. Since the early clinical symptoms of type II diabetes are not obvious, patients often delay the best time for treatment. Therefore, finding and identifying genetic markers related to type 2 diabetes in the human genome will be beneficial to the early screening and diagnosis of the disease, ...

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