PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for detecting single nucleotide polymorphism of Pax3 gene of yellow cattle and application of method

Abstract

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for detecting single nucleotide polymorphism (SNP) of a Pax3 gene of yellow cattle. The method comprises the following steps: detecting gene polymorphisms according to DNA (deoxyribonucleic acid) pool sequencing results, wherein the gene polymorphisms comprise SNP of a sequence transcription start site -580 T or G of the Pax3 gene of the yellow cattle; in the presence of a Taq DNA polymerase, a Buffer (a buffer environment), Mg<++> and dNTPs (deoxynucleoside triphosphate), performing PCR (polymerase chain reaction) amplification on the Pax3 gene of the yellow cattle, digesting a PCR amplification product by HinfI and HaeIII restriction enzymes respectively, and performing agarose gel electrophoresis on enzyme digestion segments. According to the method disclosed by the invention, a foundation is laid for creating a relationship between the SNP and the growth trait of the Pax3 gene, so that the marker assisted selection of the growth trait of the yellow cattle in China can be conveniently carried out and a new strain of high-quality special beef in China can be quickly, efficiently and accurately bred.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to the screening and detection of molecular markers of single nucleotide polymorphism (SNP) in local yellow cattle in China, in particular to a method for detecting the single nucleotide polymorphism of Pax3 gene in yellow cattle. Background technique [0002] There is still a considerable gap between the production strength of my country's beef cattle industry and the world's animal husbandry developed countries. Cultivating Chinese specialized beef cattle breeds and establishing a modern Chinese beef cattle breeding industry are strategic tasks for the development of my country's beef cattle industry. This requires the use of molecular breeding technology to improve the breeding speed and quality of beef cattle, and to accelerate the expansion of beef cattle through modern biotechnology, so as to quickly and efficiently select high-quality cattle breeds unique to my country. [00...

AI Technical Summary

Problems solved by technology

[0007] At present, there are very few studies on the Pax3 gene of cattle at home and abroad, mainly focusing on humans and mice, and most of them focus on gene function. There are no reports on the genetic variation or SNP research of Pax3 gene in livestock at home and abroad.

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