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Method, marker, and kit used for colorectal cancer diagnosis, screening, and risk prediction

A colorectal cancer and risk prediction technology, which is applied in the field of disease detection, can solve the problems of poor compliance, psychological pain, and reduced willingness of the examinee to be screened, and achieve the effect of convenient detection and low cost

Active Publication Date: 2018-07-24
太科航天智能康养技术(深圳)有限责任公司
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Problems solved by technology

The conventional gold standard for the diagnosis of colorectal cancer is colonoscopy, but it is traumatic and psychologically painful, and patients generally resist it; fecal occult blood test requires multiple sampling, which will bring a lot of inconvenience to patients, resulting in the screening of patients. Decreased willingness to investigate and poor compliance

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  • Method, marker, and kit used for colorectal cancer diagnosis, screening, and risk prediction
  • Method, marker, and kit used for colorectal cancer diagnosis, screening, and risk prediction
  • Method, marker, and kit used for colorectal cancer diagnosis, screening, and risk prediction

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Embodiment Construction

[0035] The human ribosomal protein S24 (RPS24) gene includes 6 exons, which encode ribosomal protein S24, which is a component of ribosomal protein 40s. In vitro cell studies have shown that the abnormal expression of RPS24 gene is related to the proliferation and metastasis of colorectal cancer cells, but the specific mechanism is still unclear. The present inventors have found that colorectal cancer is related to the methylation of the sequence before and after the transcription start point of the first exon of the gene RPS24. The inventor speculates that the gene RPS24 may regulate gene expression through DNA methylation, which is one of the important factors leading to the occurrence of colorectal cancer.

[0036] The present inventors have found that among the three groups of CpG in the sequence before and after the transcription initiation point of gene RPS24 as follows, group (1) and the combination of group (1) and group (2) or group (3): (1)+22, +27 and +29; (2) -24,...

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Abstract

The invention discloses a DNA methylation marker used for diagnosis, screening, and prediction of male colorectal cancer. The DNA methylation marker is obtained via methylation of a combination of thefirst groups, or the first group with the second group, or the third groups, (1), +22, +27, and +29; (2), -24,-18, +89, and +92; and (3) +136; of three CpG sites in the front and back sequence of gene RPS24 transcription start site. The sequence number is based on sequence number in SEQ ID NO.1. The 143th site transcription start site A is recorded as +1. The invention also discloses a probe, a method, and a kit used for detecting the DNA methylation marker, and a computer module used for prediction of male colorectal cancer risk using the data of the DNA methylation sites.

Description

technical field [0001] The present invention relates to the field of disease detection; more specifically, the present invention relates to a method, marker and kit for colorectal cancer diagnosis, screening and risk prediction through detection of DNA methylation in peripheral blood cells. Background technique [0002] The morbidity and mortality of colorectal cancer in my country rank fifth among all tumor morbidity and mortality respectively. For cancer, the significance of early screening far exceeds that of treatment. The conventional gold standard for the diagnosis of colorectal cancer is colonoscopy, but it is traumatic and psychologically painful, and patients generally resist it; fecal occult blood test requires multiple sampling, which will bring a lot of inconvenience to patients, resulting in the screening of patients. The willingness to check is reduced, and the compliance is poor. The hematological examination of cancer markers is non-invasive and convenient f...

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 吴安东刘长宁宁争志
Owner 太科航天智能康养技术(深圳)有限责任公司
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