3 results about "DNA Methylation Site" patented technology

The application discloses a DNA methylation site marker for detecting hepatocellular carcinoma, a multiplex dPCR kit and a diagnostic model construction method. The DNA methylation site marker comprises cg02829688, cg13080379, cg03760839, cg10703826, cg12664119, cg16990168 and cg23371746 sites. The application designs a primer probe group for the above sites, constructs a multiplex dPCR kit of a double reaction system, realizes quantification of target DNA methylation sites, takes the methylation levels of the 7 sites as characteristic variables, adopts an XGBoost algorithm and / or an LR algorithm to construct a diagnostic model. Experimental verification shows that the model can effectively distinguish hepatocellular carcinoma patients from liver cirrhosis, chronic hepatitis B, metabolic dysfunction-related fatty liver disease patients and healthy individuals, especially shows good efficiency in auxiliary diagnosis of early hepatocellular carcinoma, and provides a new technical scheme for clinical diagnosis and screening of hepatocellular carcinoma.

This invention provides a DNA methylation biomarker, kit, and device for detecting type 2 diabetes, relating to the field of disease diagnostic technology. The DNA methylation biomarker provided by this invention exhibits high sensitivity and specificity. It can be detected using the TaqMan probe method, and the detection is convenient, safe, and suitable for large-scale screening. The kit provided by this invention includes primers and probes for detecting the methylation level of the DNA methylation site cg27467803. By detecting the methylation degree of the DNA methylation site cg27467803, the risk of developing type 2 diabetes in a subject can be predicted, providing a basis for the early prediction and diagnosis of type 2 diabetes. The device provided by this invention has a simple structure and can accurately detect the risk of developing type 2 diabetes in a subject.

The application provides a DNA methylation marker cg22131907, a kit and a device for detecting type 2 diabetes, and relates to the technical field of disease diagnosis.The DNA methylation marker provided by the application has high sensitivity and strong specificity, the marker can be detected by using a Taqman probe method, and the detection is convenient, safe and easy to mass screen.The kit provided by the application comprises primer probes for detecting the methylation level of the DNA methylation site cg22131907, the incidence risk of type 2 diabetes of a subject is predicted by detecting the methylation degree of the DNA methylation site cg22131907, and basis is provided for early prediction and diagnosis of type 2 diabetes.The device provided by the application has a simple structure and can accurately realize detection on the occurrence risk of type 2 diabetes of a subject.