Method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease

A technology for resisting root-knot nematode disease and root-knot nematode is applied in the directions of botanical equipment and methods, fruit tree cultivation, plant genetic improvement, etc., and can solve the problems of tomato yield reduction, delayed grafting of rootstocks, incompatibility of rootstocks, and weak rootstocks, etc. Yield improvement effect

Inactive Publication Date: 2019-01-11
QINGDAO ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] The invention provides a method for cultivating high-affinity root-knot nematode-resistant tomato rootstocks. The method can effectively solve the problem of weak root systems of rootstocks bred by existing root-knot nematode-resistant rootstock breeding methods, and delayed grafting incompatibility of rootstocks. and characteristics, causing tomato yield reduction or even failure, making it difficult to apply in a large area in production

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  • Method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease
  • Method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease
  • Method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease

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Experimental program
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Effect test

Embodiment 1

[0018] The breeding of embodiment 1 anti-root-knot nematode tomato stock

[0019] From 2008 to 2014, nematode-resistant rootstock materials were screened for many years in a field disease nursery where tomato had been planted continuously for more than 10 years and the root-knot nematode incidence rate was 100%. Among them, the disease-resistant lines 'Z120-5-3', 'Z120-5-5' and 'Z123-2' showed vigorous plants, dark green leaves, well-developed roots, and root nematode index was 0%; 'Z05-1- 4' and 'Z05-1-5' showed vigorous plants, dark green leaves, well-developed roots, and a root nematode index of 6%. The control 'Z121' showed yellow leaves, weak plants, and a root nematode index of 61.2%. Among them, 'Z05-1-5' was the most prominent among the 12 sown lines. The plants grew vigorously, with dark green leaves, well-developed root system, and were more resistant to nematodes, while the growth of other lines was obviously not as good as that of 'Z05-1- 5', the leaves are yellow...

Embodiment 2

[0041] The identification of embodiment 2 stock grafting affinity

[0042] Previous studies have found that when '72-69' is used as a scion to graft different rootstocks, the nutrient absorption rate of the root system of the rootstock will be slower than the development rate of the scion fruit, resulting in poor compatibility. It shows that the rootstocks with good grafting compatibility will grow normally after grafting; while the rootstocks with poor grafting compatibility will grow vigorously at the seedling stage and early fruit expansion stage after grafting, but the plants will wilt or even die in the late fruit expansion stage. Phenomenon.

[0043] 2.1 Materials and methods

[0044] In the spring of 2012, grafting compatibility tests were carried out on 48 rootstock hybrid combinations using '72-69' as scion. The experiment was arranged in Qingdao Academy of Agricultural Sciences. On February 4, tomato rootstock and scion varieties were sown in seedling trays. On Ma...

Embodiment 3

[0052] Embodiment 3 verification test

[0053] Identification of Grafting Compatibility of Different Types of Scions to Rootstock ‘Z 05-1-5×Z 123-2’

[0054] 3.1 Materials and methods

[0055] In the spring and autumn of 2013, the rootstock 'Z 05-1-5×Z 123-2' was grafted with 'Qingyan Tomato No. 1' and 'Fensha No. 2' as scions respectively in the steel pipe shed of Qingdao Academy of Agricultural Sciences Product comparison test. 'Qingyan Tomato No. 1' matures early, and the fruit expands quickly. It is used as a scion. It has strict requirements on rootstocks, and rootstocks with strong compatibility can be selected. The area of ​​the cultivated plot is 3.6 square meters, the distance between plants is 0.3 meters, and the distance between rows is 0.6 meters. 20 trees are planted in each border, and repeated three times.

[0056] 3.2 Test results

[0057] 3.2.1 'Qingyan Tomato No.1' as scion

[0058] In the spring of 2013, 'Qingyan tomato No. 3' was used as rootstock, an...

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Abstract

The invention provides a method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease, which belongs to the field of tomato grafting rootstock breeding. The problem that the existing anti-root-knot-nematode rootstock breeding method causes tomato production reduction or even total crop failure and can be hardly applied in a large scale in productiondue to the weak roots, slow growth and grafting incompatibility of the rootstocks bred by the method can be solved. The technical scheme includes the following steps: selecting a specific field disease nursery to plant tomato rootstocks; evaluating the characteristics of tomato rootstocks by introducing two indexes, namely, the root development degree and the root knot nematode incidence, and breeding rootstock inbred lines with well-developed roots and resistance to root knot nematode disease; and identifying the field compatibility of the bred rootstock inbred lines through identifying scions during the whole growth period to obtain tomato rootstocks with high compatibility and resistance to root knot nematode disease. The method can be applied to the production of various tomato varieties.

Description

technical field [0001] The invention relates to a method for breeding tomato grafted rootstocks, in particular to a method for cultivating high-affinity root-knot nematode-resistant tomato rootstocks. Background technique [0002] Tomato is one of the most important vegetable crops in the world and occupies an important position in vegetable cultivation in my country. Root-knot nematode disease is an important soil-borne disease that affects tomato production. Root-knot nematodes infect tomato roots and form enlarged root knots, causing damage to the host, causing plant yellowing, dwarfing, lower leaves falling off, and small fruits at maturity. Generally, it can lead to a 20%-30% reduction in tomato production, and in severe cases, it can lead to extinction. The harm of root-knot nematode can be alleviated to a great extent by breeding tomato materials with disease-resistant genes. Since nematode resistance genes (Mi gene family) mostly exist in wild tomato species, only ...

Claims

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Application Information

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IPC IPC(8): A01G22/05A01H1/02A01H1/04A01G2/30
CPCA01H1/02A01H1/04A01G2/30A01G22/05
Inventor 黄婷婷李平张功臣刘淑芹张永志王桂莲
Owner QINGDAO ACAD OF AGRI SCI
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