31results about How to "Grafting affinity is good" patented technology

The present invention discloses the seedless litchi graft stock selecting technology. The technological process of selecting seedless litchi graft stock includes the following steps: 1. separating the simple sequence repeating (SSR) sequence through a combined selective amplifying microsatellite (SAM) and database searching method; 2. designing SSR primer with the flanking sequence of SSR sequence; 3. screening out the useful primer to obtain litchi specificity SSR marker; 4. applying the litchi specificity SSR marker in the genetic diversity analysis of partial litchi germplasm; 5. data analysis; and 6. selecting the litchi germplasm with near affinity as stock for seedless litchi grafting propagation based on the analysis result. The said process results in high survival rate and can obtain high quality seedless litchi variety.

The invention discloses a sowing method of pumpkin stock, which comprises the following steps: adopting Chinese round pumpkin in the Shandong province to cross for three generations, separating to purify, identifying to sieve self-crossing YN-3 to grow stably under low-temperature and weak light condition as mother, selecting self-crossing CN-3 of Indian long pumpkin to resist low-temperature, drought and disease as father, crossing to obtain the first-filial generation.

The invention provides a breeding method of a cold-resistant and saline-alkali-resistant grape stock. The method comprises the following steps of adopting 'river bank 580' as a female parent and 'mountain grape' as a male parent for preparing a hybrid combination, obtaining hybrid seeds in autumn of the year, carrying out sand storage stratification treatment, and sowing the hybrid seeds in the second year to obtain hybrid seedlings; after 2-3-year planting observation, selecting the hybrid seedlings strong in growth trend and free of diseases for cold resistance identification, and selectinghybrid seedlings with good cold resistance as alternatives; carrying out field cold resistance identification, saline-alkali resistance identification and grafting compatibility experiments on the hybrid seedlings selected in step 2 to obtain the grape stock. The grape stock bred by means of the method has the advantages of high cold resistance and saline-alkali resistance, good grafting affinityperformance, strong growth potential, straight and smooth branches, few bad branches and high maturity, and the strip yield can reach 10,250 meters per mu.

The invention belongs to the technical field of breeding of cucurbitaceae vegetable. An interspecific hybrid F1 (Yong rootstock No. 6) of cucurbit (a melon rootstock) is obtained by hybridizing a female parent of a stable inbred line J03-2-3-2-1-5-7-4 by multi-generation of directed autocopuation screening of cucurbit materials originated from Japan and a male parent of a stable inbred line YH2-3-3-2-1-4-2-6 by multi-generation of directed autocopuation screening of local Lagenaria species produced in Ningbo. The melon rootstock selectively bred with the method provided in the invention meets objectives of breeding and has moderate growth potential, high resistance to die-back and root rot, well-developed roots, strong stalks, strong ability in absorbing nutrient, low degree of vain growth and robust hypocotyls which are not easy to become hollow, and therefore the melon rootstock is favorable for grafting. The melon rootstock has good grafting affinity and strong symbiosis affinity, and a survival rate over 95% is obtained for the grafting of the rootstock onto the watermelon specie ''Zaojia'' (8424). The engrafted melon rootstock is low temperature-resistant, moisture-resistant and barren-resistant; after field planting, the root and overground part of a grafted seedling grows fast and has sound growth potential, and the period of first harvest of the grafted seedling is 5 days earlier than that of a self-rooted seedling, the fruition rate is high and stable, weight of single fruit and fruit shape can substantially maintain the same, melon skin is not thickened, sugar degree of the grafted seedling is higher than that of the self-rooted seedling, sugar degree gradient is small, quality of the watermelon is not affected, and good adaptability is obtained.

The invention discloses a cultivation method of rosa rugosa vat trees, and belongs to the field of garden plant cultivation. The cultivation method of the rosa rugosa vat trees comprises the followingsteps of cultivating rosa multiflora into shaping rootstocks, grafting rosa rugosa vat scions, and conducting management after grafting; or cultivating the rosa multiflora into the shaping rootstocks, conducting mixed grafting of the rosa rugosa vat scions and rosa rugosa Thunb scions, and conducting management after grafting. According to the method, the rootstock and scion grafting combination,the grafting period and the rootstock heading height are optimized, it is found that American floriferous and thorn-free rosa multiflora serves as rootstocks and has the best grafting affinity with pink single-petal roses; when grafting is conducted on the middle ten days of March, the grafting survival rate is the highest; when the rootstock heading height is 85-95 cm, the ornamental effect is the best. The rosa rugosa vat trees cultivated by means of the method have independent trunks, the tree shapes are full and attractive, and both flowers and fruits can be appreciated; the rosa rugosa vat and rosa rugosa Thunb are subjected to mixed grafting to the same rootstocks, the flower appreciation effect can be further improved, the flowering time can be prolonged, and the ornamental value and commercial value of the rosa rugosa vat trees are increased.

The invention provides a Kingson privet spherical crown rapid forming method. Ligustrun lucidum is used as a stock and Kingson privet is used as a scion to carry out grafting and cultivating, and the Kingson privet is obtained. According to the method, the Ligustrun lucidum with a fast growth rate is used as the stock for grafting the Kingson privet, cost is low, efficiency is high, and obtained plant is good in the ornamental effect.

The invention discloses a breeding method of hybridization stake specially for prevention and cure of cucurbit wilt comprising, selecting the Zhejiang local long gourd variety for autocopulation, self-detaching and purification of three generations, evaluating inbred line CH1 exhibiting stable strain growth under low-temperature and low irradiance and having strong low temperature resistance as mother parent, selecting the Zhejiang local long round variety for autocopulation, self-detaching and purification of three generations, evaluating and screening inbred line YH-6 exhibiting strong low-temperature and heat resistance as father parent, preparing genetics F1 hybrid which is named 'Hulu Zhan No. 1'.

The invention belongs to the technical field of semievergreen street tree cultivation and discloses a method for cultivating tassel-like lonicera japonica based on lonicera maackii grafted lonicera japonica and lonicera hypoglauca. The method includes: cultivating lonicera maackii seedlings by taking lonicera maackii seeds as propagating materials in a breeding field, transplanting annual lonicera maackii seedlings into field, and maintaining single poles after base stumping in spring in the second year for cultivating high-trunk arbor trees; selecting annual lonicera japonica and lonicera hypoglauca as scions, and keeping two pairs of buds for scions; adopting a bark grafting or split grafting method for grafting; observing germination condition of the scion buds two weeks after grafting, judging survival condition of grafting, and removing bindings in time after the scions become branches; trimming the trees at right time after the scions survive and become branches, and cutting off lateral branches of the trunks of lonicera maackii to promote quick growth and shaping of lonicera japonica and lonicera hypoglauca. Novel semievergreen ornamental street trees are cultivated with the grafting technique.

The invention discloses a planting method of Korla fragrant pear. It belongs to the technical field of agriculture, forestry and fruit industry. The method comprises the following steps: planting rootstocks: planting rootstocks in the spring of the first year; grafting an intermediate stock "Xinli No. 9" on the rootstocks in the spring of the second year; grafting Korla fragrant pears in the spring of the third year. Compared with the prior art, the present invention has the following beneficial effects: using the variety "Xinli No. 9" as the dwarfed interstock of Korla fragrant pear, compared with other dwarfed interstocks, it has good grafting affinity, and is suitable for grafting. After that, there is no "big foot" phenomenon, and it has a certain dwarfing performance for Korla fragrant pear, which is manifested in that the growth of branches is only 3/4 of that of Korla fragrant pear directly grafted with Du pear as the rootstock; at the same time, it has strong cold resistance. , suitable for promotion and application in the colder southern Xinjiang, suitable for controlling the vigorous growth of Korla fragrant pear varieties, and conducive to early fruiting of pear trees.

The invention discloses a rootstock selecting method for domestic mainly-planted varieties of cerasus. The rootstock selecting method includes the following steps that S1, the domestic main varietiesare selected, and scion materials are further selected; periphery, middle and upper branches of the annual different domestic main varieties of cerasus are selected and used as grafting scions; S2, the scions are treated, and the scions are collected when the cherry blossoms of the cerasus do not yet bud and refrigerated at low temperature; S3, rootstocks are selected, and the selected rootstock species include Cerasus serrulata rootstocks, Prunus conradinae rootstocks, and Prunus dielsiana Schneid rootstocks; S4, a grafting operation is carried out when the rootstocks begin to sprout; S5, management is carried out after grafting, and in the later period, after incisions are healed, bandages can be removed; and S6, the grafting success rate of different domestic mainly-planted varieties ofcerasus on different types of rootstocks is counted and recorded. The rootstock selecting method for the domestic mainly-planted varieties of cerasus is simple and easy to operate.

The invention provides a method for cultivating tomato rootstocks with high compatibility and resistance to root knot nematode disease, which belongs to the field of tomato grafting rootstock breeding. The problem that the existing anti-root-knot-nematode rootstock breeding method causes tomato production reduction or even total crop failure and can be hardly applied in a large scale in productiondue to the weak roots, slow growth and grafting incompatibility of the rootstocks bred by the method can be solved. The technical scheme includes the following steps: selecting a specific field disease nursery to plant tomato rootstocks; evaluating the characteristics of tomato rootstocks by introducing two indexes, namely, the root development degree and the root knot nematode incidence, and breeding rootstock inbred lines with well-developed roots and resistance to root knot nematode disease; and identifying the field compatibility of the bred rootstock inbred lines through identifying scions during the whole growth period to obtain tomato rootstocks with high compatibility and resistance to root knot nematode disease. The method can be applied to the production of various tomato varieties.

The invention provides a method for cultivating a high-affinity fusarium wilt-resistant tomato rootstock, and belongs to the field of tomato grafting rootstock breeding. The problems of yield reduction or even no harvest and difficult large-area application in production of tomatoes, caused by weak roots, slow growth and grating non-affinity of rootstock bred by existing fusarium wilt-resistant rootstock breeding methods are solved in the invention. The method comprises the following steps: selecting a specific field disease nursery, planting tomato rootstocks, evaluating the characteristics of the tomato rootstocks by introducing two indexes of root development degree and fusarium wilt-resistant incidence, selecting a rootstock inbred line having developed roots and having resistance to fusarium wilt, and carrying out field affinity identification on the bred rootstock inbred line in the whole growth period by identifying scions in order to obtain the high-affinity fusarium wilt-resistant tomato rootstock. The method can be applied to the production of various varieties of tomatoes.