A kind of breeding method of small-seed type pumpkin rootstock variety
A variety and rootstock technology, which is applied to the field of breeding of small-seeded pumpkin rootstock varieties, can solve the problems of increasing the difficulty of breeding small-grain varieties, unclear genetic laws of pumpkin seeds, etc.
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Embodiment 1
[0041] The breeding method of embodiment 1 small-seed type pumpkin rootstock variety
[0042] One, the breeding method of the small-seed type pumpkin stock variety of the present invention, comprises the following steps:
[0043] (1) Acquisition of genetic laws
[0044] Through the investigation of pumpkin germplasm resources, combined with the breeding objectives, two materials NMO83 and XBZM with significant differences in grain size were selected, and their thousand-grain weights were 110g and 160g respectively; the two were crossed as parents to obtain hybrids F1 and RF1, Then F1 was self-crossed to obtain the F2 generation, and F1 was backcrossed with NM083 to obtain the BC1 generation; NMO83, XBZM, F1, RF1, F2 and BC1 were planted at the same time in the next season; the thousand-grain weight of the seeds was inspected after the fruit matured, and the results of F1 and RF1 The thousand-grain weights of pumpkin were 178g and 214g, which were relatively close to the large...
Embodiment 2
[0113] Comparison test of the small-seed pumpkin rootstock variety 'Si Zhuang No. 10' that was obtained through breeding in Example 2
[0114] The following is the summary of the comparative test results of the 'Sizhuang 10' variety from 2016 to 2017
[0115] 1 Test overview
[0116] In order to investigate and identify the adaptability, disease resistance, grafting performance, watermelon quality and yield of the newly selected small-seeded pumpkin rootstock variety 'Sizhuang 10', the rootstock 'All-round Tiejia' (CK1) and The self-rooted seedlings of 'Zaojia' (CK2) were used as the control. From 2016 to 2017, multi-year multi-point experiments were carried out. The test sites are Ningbo High-tech Agricultural Technology Experimental Park (Ningbo), Pinghu Lvye Fruit and Vegetable Professional Cooperative (Pinghu) and Deqingtian Agricultural Technology Co., Ltd. (Deqing).
[0117] 2 Results and Analysis
[0118] 1) Phenological period
[0119] Table 1 Overview of phenolog...
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