The invention discloses a grafting and cultivation method of Taxus chinensis, and belongs to the technical field of traditional Chinese medicine material planting. The method includes preparation before grafting, grafting operation and management after grafting. The preparation before grafting, comprises the steps that grafting time is June to July, a scion is collected 15-30 d before grafting, the whole scion is sealed with paraffin and is put into wet sand in a cool place for preservation. The rafting operation comprises the steps of a, treatment of cutting scion, wherein the grafting opening is dipped in a root growing foliar fertilizer for standby application; b, grafting, wherein stopping cutting at the grafting part, one side of a rootstock section is cut into a concave triangular rootstock groove using a grafting knife, the scion is taken out, the other end of the rootstock section is cut into a convex triangle corresponding the concave triangle rootstock groove, the scion is embedded into the rootstock groove, both sides of the rootstock groove are made to be adjoined, and a callus anti-corrosion film is smeared. The management after grafting comprises the steps of a, unbinding and removing burgeons; b, applying the fertilizer and moisture management; c, grafted seedling pest control. According to the grafting and cultivation method of Taxus chinensis, grafting is conducted in June to July, the method is suitable for grafting of a big rootstock, and has the advantages of being fast in shooting, early in formation of crowns, smooth in grafting healing part and high in grafting survival rate.