Oil-tea SSR molecular marker

A molecular marker, Camellia oleifera technology, which is applied in the development of Camellia oleifera microsatellite genetic markers, can solve the problems of little research on the development and utilization of Camellia oleifera microsatellite molecular markers, and achieves high reliability and good repeatability.

Active Publication Date: 2014-03-26
JIANGXI ACAD OF FORESTRY
View PDF1 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been little research on the development and utilization of camellia oleifera microsatellite molecular markers, and there are only a few domestic reports on the use of tea tree microsatellite marker primers to randomly amplify the camellia oleifera genome (Liu Bing et al., Establishment and optimization of Camellia oleifera SSR-PCR reaction system. Journal of Anhui Agricultural University ,2011,38(6)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oil-tea SSR molecular marker
  • Oil-tea SSR molecular marker
  • Oil-tea SSR molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Preparation method of camellia oleifera SSR molecular marker

[0062] 1.1 Acquisition and pretreatment of Camellia oleifera DNA sequence

[0063] Using the method of our research group (refer to Jiang C et al. Efficient extraction of RNA from various Camellia species rich in secondary metabolites for deep transcriptome sequencing and gene expression analysis. African Journal of Biotechnology, 2011, 10(74): 16769-16773. ) to extract Camellia oleifera DNA and RNA, construct genome and transcriptome libraries respectively, and obtain genome and EST sequences through each 1 / 4 454GS FLX high-throughput sequencing reaction. The EST sequence was spliced ​​by CAP3 after removing low-quality sequences by SeqClean and Lucy software, and the genome sequence obtained by sequencing was processed and spliced ​​by the Newbler software that comes with the 454 sequencer.

[0064] 1.2 Screening of SSR loci in Camellia oleifera DNA sequence

[0065] The SSR sites in each DNA sequence...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an oil-tea SSR molecular marker which comprises acquisition and pre-treatment of an oil-tea DNA sequence, retrieval of SSR loci in the DNA sequence, design and synthesis of an SSR primer, establishment of an SSR marker system, and screening of a polymorphic SSR marker. Oil-tea genomic sequences obtained by 454 sequencing and eligible sequences containing simple sequence repeat units in an EST sequence are retrieved and screened to obtain twelve polymorphic SSR markers: CO_gSSR11, CO_gSSR12, CO_gSSR14, CO_gSSR16, CO_gSSR19, CO_gSSR20, CO_eSSR01, CO_eSSR04, CO_eSSR16, CO_eSSR40, CO_eSSR44 and CO_eSSR48. The oil-tea SSR molecular marker disclosed by the invention is suitable for genetic evaluation, germplasm identification and molecular marker assisted breeding research of oil-tea germplasm resources, has the advantages of good repeatability and high reliability, and is an effective molecular marker.

Description

technical field [0001] The invention relates to a DNA molecular marker technology, in particular to the development technology of microsatellite genetic markers for camellia oleifera, which is suitable for genetic diversity research, germplasm identification and kinship analysis of camellia oleifera. Background technique [0002] Microsatellite (Microsatellite) refers to a DNA sequence that is repeated in series with a few nucleotides (mostly 2-4) widely distributed in the genome, which is called simple sequence repeats (Simple sequence repeats) , SSR), is a type of DNA sequence with a relatively high mutation rate in the genome. In recent years, SSR has become the most popular genetic marker. As a co-dominant marker with high detection efficiency, SSR markers combine the advantages of other molecular markers, such as typical co-dominant and multi-allelic genes, with high polymorphism, and can accurately distinguish individuals with very similar kinship; The amount of DNA ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C12Q1/68
Inventor 温强刘丽婷徐立安徐林初周文才叶金山朱恒黄敏仁
Owner JIANGXI ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap