Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof

A high-strength fiber and molecular marker technology, applied in the field of molecular genetic breeding, can solve problems such as difficulty, slow progress, and high cost of quality breeding, and achieve the effect of improving fiber quality and selection efficiency

Active Publication Date: 2013-08-21
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Therefore, the cost of quality breeding is high, difficult and slow

Method used

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  • Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof
  • Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof
  • Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof

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Embodiment 1

[0031] The implementation procedure of the present invention is: the upland cotton high-strength fiber germplasm line Prema that introduces from the U.S. is carried out individual plant selection, and measure fiber quality, select the homozygous stable strain Prema, according to the measurement result in 2000 and 2001, The average fiber quality of strain Prema in two years is: fiber strength 35.63cN / tex, fiber length 30.39mm, micronaire value 4.25, upland cotton variety 86-1 (a fusarium wilt resistant variety bred by researcher Ma Cun of the Chinese Academy of Agricultural Sciences) : Fiber strength 25.70cN / tex, fiber length 27.74mm, micronaire value 4.72. The above two germplasm materials have been publicly distributed free of charge, and are widely used in Chinese cotton genetics and breeding institutions. At the same time, if other colleagues need it, the Cotton Research Institute of Nanjing Agricultural University can provide these germplasms to domestic research instituti...

Embodiment 2

[0044]

[0045]

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Abstract

The invention discloses a major QTL (Quantitative Trait Locus) of a cotton high-strength fiber and a molecular marker and application thereof. The major QTL of the cotton high-strength fiber is linked with two SSR (Simple Sequence Repeat) markers, is positioned through markers NAU / SSR / FS1195 and JESPR / SSR / FS2127 and is 0.01-2.39 centimeters away from a marker NAU3700; and the major QTL locus can explain 4.51%-17.55% of phenotype variation and achieves the LOD (Limit Of Detection) value between 3.23 and 7.09. The major QTL disclosed by the invention overcomes the defects of high cost, high difficulty and slow progress of quality breeding in the prior art, greatly increases the selection efficiency of the high-strength fiber by utilizing the major QTL locus of a cotton high-strength fiber gene and the molecular marker thereof, can be used for exploring high-quality fiber gene resources, provides the gene resources and a marker auxiliary selection technology for the quality breeding of cotton and can be applied to the production and quality detection of high-strength fiber cotton varieties.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and relates to a cotton high-strength fiber main effect QTL, molecular markers and applications thereof. technical background [0002] Cotton fiber is an important raw material for the textile industry. With the continuous innovation of textile technology to greatly reduce costs and improve textile efficiency, air rotor spinning, air-jet spinning and friction spinning technologies are rapidly eliminating old-fashioned ring spinning technologies. These new textile technologies have great impact on fiber Quality has higher requirements. Therefore, there is an urgent need to rapidly improve the quality of cotton fiber in my country. In the past 15 years, the output of my country's self-bred cotton varieties is slightly higher than that of the United States, but the fiber quality is somewhat different. The fiber quality of my country's leading varieties is medium, which can basically meet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C12N15/82A01H5/00
Inventor 张天真宁志怨郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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