Method for identifying purity of tobacco variety Zhongyan 90 by using specific molecular marker method

A technology of molecular markers and variety purity, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of narrow genetic background, limited genetic information, and limited polymorphism of flue-cured tobacco varieties, and achieve intuitive detection results , high detection efficiency and simple operation

Inactive Publication Date: 2013-03-13
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the cost of this type of detection technology is relatively high, and the amount of genetic information is limited, coupled with the narr

Method used

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  • Method for identifying purity of tobacco variety Zhongyan 90 by using specific molecular marker method
  • Method for identifying purity of tobacco variety Zhongyan 90 by using specific molecular marker method
  • Method for identifying purity of tobacco variety Zhongyan 90 by using specific molecular marker method

Examples

Experimental program
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Embodiment 1

[0023] Example 1 Determining the characteristic band of China Tobacco 90

[0024] 1. The test materials are shown in Table 1:

[0025] Table 1 Test materials

[0026]

[0027]

[0028] 2. Sow the seeds of various test materials to be determined in Table 1 on the seedling tray in the light incubator, and wait for the plants to grow until the genomic DNA can be directly extracted.

[0029] 3. Extract the genomic DNA of the tobacco variety to be tested (using the CTAB / NaCl method):

[0030] Take about 0.2g of a young leaf of each test material, put it in a mortar, pour liquid nitrogen into it and quickly grind it into powder, and put it into 1.5ml of 0.7ml 65°C preheated CTAB extraction buffer. In a centrifuge tube, oscillate quickly to mix well, and place in a water bath at 65°C for 1 hour, during which time shake well; place it at room temperature, add an equal volume (0.7ml) of chloroform-isoamyl alcohol (v / v 24:1), and mix gently. 4°C, 8000r, centrifuge for 10min; ta...

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Abstract

The invention relates to a method for identifying the purity of a tobacco variety Zhongyan 90 by using a specific molecular marker method, wherein the purity of the flue-cured tobacco variety Zhongyan 90 is identified by using an SSR (Simple Sequence Repeats) molecular marker. According to the invention, based on a large amount of experiments, a specific primers of a characteristic strip of Zhongyan 90 are obtained through amplification, the specific primer sof Zhongyan 90 are used to carry out purity detection, detection results are reliable and visual, and compared with the conventional detection method, the method disclosed by the invention is simple to operate, high in detection efficiency and lower in cost. With adoption of the method, the detection period is short, that is, the purity of a seed can be detected in a seedling stage by using the method, in addition, standards are unified and are not influenced by human factors and environment, and results are accurate and reliable.

Description

(1) Technical field [0001] The invention relates to a method for identifying the purity of tobacco "Zhongyan 90" by a specific molecular marker method, and belongs to the field of tobacco breeding and biotechnology. (2) Background technology [0002] Seeds are the most basic means of production in agriculture, and the quality of seeds directly affects the yield and quality of crops. At present, the main tobacco varieties are mixed and the seed degradation is relatively serious, which has brought huge economic losses to farmers. Carrying out seed purity inspection is of great significance to ensure seed quality and improve tobacco production efficiency. [0003] Seed purity is one of the important indicators for evaluating the quality of tobacco seeds. At present, the methods for identifying the purity of tobacco seeds used in my country are mainly conventional identification methods. Among them, conventional identification methods mainly include seed morphology identificat...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杨龙黄莉莎王玉军曹恒春王毅
Owner SHANDONG AGRICULTURAL UNIVERSITY
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