Method for controlling quick propagation light source for upland cotton

A light source control, upland cotton technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as low light efficiency, and achieve the effects of good effect, simple operation, energy saving and environmental protection

Inactive Publication Date: 2010-09-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the spectral energy distribution of these light sources is designed and produced based on the principle of high-eff

Method used

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  • Method for controlling quick propagation light source for upland cotton
  • Method for controlling quick propagation light source for upland cotton
  • Method for controlling quick propagation light source for upland cotton

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Embodiment Construction

[0016] The methods used in the following examples are conventional methods unless otherwise specified, and the percentages are volume percentages unless otherwise specified.

[0017] At first carry out light-emitting diode light treatment to Su cotton 22 (kind of production) with the method of the present invention, comprise following three steps:

[0018] 1) Preparation of explants:

[0019] 1.1) Take the seeds of Sumian 22, the main cotton upland cotton variety in my country that has been delinted with concentrated sulfuric acid, and wash them in running water for 2-3 hours, then disinfect them with 75% alcohol for 30 seconds in an ultra-clean workbench, and use 15% sodium hypochlorite for 10 seconds. -15 minutes, 15% hydrogen peroxide for 10-15 minutes, rinse with sterile water 5-6 times, soak the sterilized seeds in sterile water for 12-24 hours;

[0020] 1.2) Then use tweezers to pick and peel off the seed coat, insert the seed kernel into MS medium, culture it in the dar...

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Abstract

The invention discloses a technique for controlling a quick propagation light source for upland cotton, which belongs to the technical field of biology. The technique comprises the following steps of: flushing upland cotton seeds by using running water, sterilizing the flushed seeds in a clean bench, and soaking the sterilized seeds in sterile water for 12 to 24 hours; peeling off seed husks by using forceps, inoculating seed kernels to an MS culture medium, culturing the seed kernels in dark for 2 days, then culturing the seed kernels under fluorescence lamp light for 5 days, and taking apical buds serving as explants after the seed leaves are flattened; cutting about 2 centimeters of apical buds with partial hypocotyls, culturing the cut apical buds under a fluorescence lamp for 5 days, and then culturing the cut apical buds for 40 days under different light sources of fluorescence lamps (contrast) in a ratio of monochrome blue light to red light and blue light of 3 to 1, a ratio of red light to blue light of 1 to 1 and a ratio of red light to blue light of 1 to 3. The method has the advantages of simple operation, quick growth and robustness of plants, energy conservation, environmental protection and the like, and has high practical application value.

Description

1. Technical field [0001] The invention relates to a method for controlling a fast-growing light source of upland cotton, belonging to the field of biotechnology. 2. Background technology [0002] Light sources used in plant cultivation are generally fluorescent lamps, metal halide lamps, high pressure sodium lamps and incandescent lamps. However, the spectral energy distribution of these light sources is designed and produced based on the principle of high-efficiency demand for light by the human eye, which contains many unnecessary wavelengths and the light efficiency is very low. Compared with traditional light sources, light-emitting diodes, as a new type of semiconductor light source that can be used for plant illumination, have attracted increasing attention in recent years. The basic structure of the LED is a piece of electroluminescent semiconductor material, which is placed on a shelf with leads and sealed with epoxy resin around it to protect the inner core wire. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李慧敏徐志刚唐灿明
Owner NANJING AGRICULTURAL UNIVERSITY
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