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Method for culturing cotton resisting micro-bundle disease

A disease and cotton technology, applied in the field of cultivating cotton resistant to vascular bundle diseases, can solve the problems of great influence, difficulty in prevention, and difficulty in popularization and application, and achieve the effects of high grafting survival rate, promotion of healthy development, and promotion of construction process

Inactive Publication Date: 2010-02-10
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verticillium dahliae is a soil-borne fungus. It enters the xylem through the cotton root infection and is transported to the aboveground part of the plant through the xylem transport system, causing Verticillium wilt symptoms. At present, there are almost no real stable resistant fungi in production. sex breed
The reasons for the difficulty in control are: (1) there is a lack of Verticillium wilt-resistant genes in upland cotton germplasm resources, and the Verticillium wilt-resistant genes in sea-island cotton are difficult to be applied. Therefore, the selection and breeding of upland cotton varieties resistant to Verticillium wilt has been restricted; (2) Cotton Verticillium dahliae can survive for a long time on crop residues or even directly in the soil for more than ten years in the form of its dormant structure-microsclerotia, and the amount of bacteria can accumulate year by year. Once encountering suitable conditions, it will cause the outbreak of verticillium wilt; (3) the effect of chemical control has been poor, and pollute the environment, which is easy to destroy the ecological balance of farmland; (4) agricultural control measures, such as stubble, crop rotation, and more organic fertilizers etc. Although there are certain effects, under the unique agricultural environment conditions and farming patterns in my country, it is difficult to popularize and apply; (5) Although biological control has great potential, it currently lacks efficient antagonistic bacteria, and there are The control effect is not complete, and the antagonistic bacteria are greatly affected by the environment (temperature, humidity, moisture, etc. in the soil) in the field

Method used

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  • Method for culturing cotton resisting micro-bundle disease
  • Method for culturing cotton resisting micro-bundle disease
  • Method for culturing cotton resisting micro-bundle disease

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 A method of cultivating soil-resistant vascular disease cotton, comprising the following steps:

[0033] (1) Raising rootstock The sea-island cotton strain Hai 7124 was selected as the rootstock, and its cotton seeds were sterilized and sown in paper pots with substrates. Two cotton seeds were sown in each pot and placed in the greenhouse.

[0034] (2) Scion breeding The susceptible upland cotton line Jimian 11 was selected as the scion, and the selected cotton seeds were sterilized and sowed in paper pots equipped with substrates. Three cotton seeds were sown in each pot and placed in the greenhouse.

[0035] The cotton seed disinfection treatment method is: after soaking in 75% alcohol for 1 minute, wash 4 times with sterilized double water, then soak in 0.5% HgCl 2 After incubation for 5 minutes, wash 7 times with sterile double water.

[0036] The preparation method of the above-mentioned nutrient paper pot is as follows: mix vermiculite and sand after...

Embodiment 2

[0039] Embodiment 2 is a method for cultivating cotton resistant to soil-borne vascular diseases, which is basically the same as in Embodiment 1, except that the sea-island cotton variety Pima S-7 is selected as the rootstock.

Embodiment 3

[0040] Example 3 A method for cultivating soil-borne vascular disease-resistant cotton is basically the same as Example 1, except that the F 1 Substitute rootstock.

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Abstract

The invention relates to a method for culturing cotton resisting a micro-bundle disease. The method comprises the following steps: using sea island cotton or land-sea hybrid cotton with high resistance and immunity to the greensickness and the blight of a main cotton micro-bundle disease as a parental stock; using a general upland cotton specie or strain as scion; grafting at a seedling stage; andculturing grafted seeds in an environment with the temperature of 25-35 DEG C, the relative humidity of 85-95 DEG C and indirect lighting for 5-10 days. The method selects the sea island cotton or the land-sea hybrid cotton as the parental stock and inhibits or prevents micro-bundle pathogens from expanding under the effect of physical obstacle (texture structure) or chemical inhibition thereof or interaction the physical obstacle (texture structure) or the chemical inhibition thereof so as to present immunity or high resistance to the greensickness and the blight of a main cotton micro-bundle disease. The method has high grafting survival rate (higher than 90 percent), grafting cotton has strong advantages, high yield, longer stem than non-graft scion, larger fruit branch number than thenon-graft scion and strong-growth characteristic similar to the parental stock.

Description

technical field [0001] The invention relates to the field of cotton cultivation, in particular to a method for cultivating cotton resistant to vascular bundle diseases. Background technique [0002] The annual cotton planting area in my country is about 80 million mu. However, due to the long-term lack of effective control measures or improper control measures, serious cotton diseases often break out in my country's cotton fields, often causing incalculable losses. Cotton diseases mainly include cotton soil-borne microvascular diseases Verticillium wilt and Fusarium wilt, cotton seedling diseases (including blight, blight and anthracnose, etc.) and cotton boll rot. The ecological environment of the main cotton areas in my country is different, and the occurrence and damage degree of the main diseases are also different. In most years, the area of ​​occurrence and yield loss caused by cotton microtubule diseases Verticillium wilt and cotton wilt are much higher than those of...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G1/06
Inventor 郝俊杰贾新合朱和琴马平贺兆伟胡雨薇
Owner HENAN ACAD OF AGRI SCI
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