The invention discloses a breeding method of a high-quality three-line sterile line I2A. Artificial hybridization is carried out by using D-
aroma 1B as a female parent and gold 23B as a male parent, asingle
plant with an excellent
plant type, excellent comprehensive traits and resistance to rice blast is bred through three-generation systematic breeding, then the
single plant and Gang 46A are subjected to multi-generation continuous
backcrossing, in the
backcrossing process, microscopic examination and selection are carried out generation by generation, a
single plant which is completely abortive in
fertility and has the
plant type basically the same as that of the male parent is selected from a sterile line
backcrossing group and backcrossed with the male parent, two-way selection is carried out, the breeding cycle is shortened, and the high-quality three-line sterile line which has stable
fertility, excellent rice quality, a high
reproduction and seed production yield, good resistance of a matched combination, high
hybrid vigour and good comprehensive traits is finally bred and named as the high-quality three-line sterile line I2A and does not have
aroma. The matched combinationof the high-quality three-line sterile line I2A is excellent in rice quality and high in yield, the organic combination of the high quality and the high yield is truly achieved, and the rice breedingproblem that
hybrid rice is high in yield but not high in quality is solved.