Gossypium hirsutum GhB2 protein as well as encoding gene and application thereof

A technology encoding genes and proteins, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of loss of resistance, lack of genetic resources for resistance to Verticillium wilt of upland cotton, loss of agricultural production, etc.

Inactive Publication Date: 2016-07-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, upland cotton lacks genetic resources for Verticillium wilt resistance, and the main disease-resistant gene has not been cloned in cotton, and the resistance mediated by the disease-resistant gene is onl

Method used

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  • Gossypium hirsutum GhB2 protein as well as encoding gene and application thereof
  • Gossypium hirsutum GhB2 protein as well as encoding gene and application thereof
  • Gossypium hirsutum GhB2 protein as well as encoding gene and application thereof

Examples

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Embodiment 1

[0061] Example 1. Acquisition and functional verification of GhB2 gene in upland cotton

[0062] 1. Acquisition of GhB2 gene in upland cotton

[0063] 1. Planting of cotton materials:

[0064] Cotton (the cotton varieties are Zhongzhi Cotton KV3 and 86-1 (source see above), Gossypium hirsutum L.)) seeds were delinted with sulfuric acid. Select plump cotton seeds, soak them in 70% alcohol for 5 minutes, and then use 5% H 2 o 2 Soak for 2 hours, rinse with sterile water for 3 times, then soak in sterile water for 5 hours to accelerate germination. The seeds were planted in flower pots, and the test soil was sterilized vermiculite. Culture conditions: 26 / 20°C, light for 16 hours, and darkness for 8 hours. After the cotton plant grows cotyledons, move to a medium containing 1 / 3 MS culture solution (see literature: MurashigeTandSkoogF.Arevisedmediumforrapidgrowthandbioassayswithtobaccotissuecultures.PhysiolPlant, 1962,15:473-497.) Cultivate in a large beaker until the second t...

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Abstract

The invention discloses gossypium hirsutum GhB2 protein as well as an encoding gene and application thereof. The GhB2 protein provided by the invention is as shown in (1) or (2): (1) proteins as shown in SEQ ID No. 2; (2) proteins obtained through substitution and/or deficiency and/or addition of one or more amino acid residues on amino acid sequences shown in the SEQ ID No. 2, and with the same functions. According to the gossypium hirsutum GhB2 protein and the encoding gene and application thereof disclosed by the invention, sequencing of a transcription group of Chinese planted cotton KV3 is used as a foundation, cotton GhB2 genes are cloned, typical DCD ultra-protein family genes are obtained, procedure death of cells is mediated, through gene silencing and overexpression analysis, the effects of the gossypium hirsutum GhB2 protein in verticillium wilt resistance for Gossypium hirsutum are discussed, and candidate genes are provided for subsequent cultivation of disease-resistant varieties through genetic engineering means.

Description

technical field [0001] The invention relates to the field of biology, in particular to the upland cotton GhB2 protein and its encoding gene and application. Background technique [0002] my country is one of the largest cotton-producing countries in the world. As an important raw material for textile, chemical, pharmaceutical and national defense industries, cotton occupies an important position in the national economy. Verticillium dahliae (Verticillium dahliae Kleb.) caused by Verticillium dahliae (Verticillium dahliae Kleb.) is a soil-borne fungal vascular disease. Since it was discovered in the United States in 1914, it has spread to various cotton-producing countries in the world, and it has affected my country and the world's cotton. One of the most important diseases of production, known as the "cancer" of cotton. Especially after the 1990s, cotton verticillium wilt has been prevalent in a large area continuously in my country, and the diseased area accounts for more ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8282
Inventor 张文蔚张华崇简桂良齐放军
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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