A molecular breeding method for simultaneous improvement of cotton Verticillium wilt resistance, fiber quality and yield traits

A cotton Verticillium wilt and resistance technology, applied in the field of cotton molecular breeding, can solve the problems of slow breeding progress, high cost, long cycle, etc., and achieve the effect of improving Verticillium wilt resistance, accelerating the process of cultivation and seed industrialization

Active Publication Date: 2021-11-23
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0008] In order to overcome the many shortcomings of traditional breeding such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost, and solve the problems of synchronous improvement of Verticillium wilt resistance, fiber quality and yield traits and slow breeding progress

Method used

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  • A molecular breeding method for simultaneous improvement of cotton Verticillium wilt resistance, fiber quality and yield traits
  • A molecular breeding method for simultaneous improvement of cotton Verticillium wilt resistance, fiber quality and yield traits

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1 Screening Molecular Markers

[0041] (1) Construction of introgression lines and acquisition of phenotypic data

[0042] Using Haidao Mianhai 1 as the donor parent and Zhongmian 36 as the recurrent parent, a high-generation backcross introgression line population of land-sea hybrids was constructed. The recurrent parent Zhongmian Research Institute 36 (CCRI36) is an early-maturing and excellent upland cotton variety (National Approved Cotton 990007) cultivated by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences, while the donor parent Hai1 (Hai1) is a dominant aglandular A sea-island cotton strain with high resistance to Verticillium wilt and excellent fiber.

[0043] In the summer of 2003, in Anyang, Henan Province, F 1 , in the winter of the same year, BC was obtained by backcrossing 36 as the female parent 1 f 1 ; Afterwards, Zhongmian 36 was used as the recurrent parent and continuously backcrossed to BC 5 f 1 , and then ...

Embodiment 2

[0062] Example 2 Molecular marker selection-assisted breeding for simultaneous improvement of cotton Verticillium wilt resistance, fiber quality and yield traits

[0063] Using the molecular marker NAU3665 obtained in Example 1 240bp Carry out molecular marker selection in the breeding population related to Sea Island Cotton Sea 1 and its derived strains (variety), comprising the following steps:

[0064] (1) DNA extraction: Sea Island Cotton Sea 1 and its derived strains (variety) were used as donor parents, and upland cotton varieties or strains (such as Zhongmian Institute 60, Lumianyan 28, and Xinluzao 60) were used as recipient parents, Perform hybridization and backcrossing to obtain isolated populations, and use the CTAB method to extract DNA from individual plants of the isolated populations at the seedling stage;

[0065] (2) Using the molecular marker NAU3665 240bp Carry out molecular marker detection to the genotype of the above-mentioned (1) population individual...

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Abstract

The invention relates to the technical field of molecular breeding, and discloses a molecular breeding method for synchronously improving cotton verticillium wilt resistance, fiber quality and yield traits. The molecular breeding method for simultaneous improvement of cotton Verticillium wilt resistance, fiber quality and yield traits, the SSR molecular marker used is NAU3665 240bp . The present invention helps to overcome many deficiencies such as poor selection accuracy, low efficiency, long cycle and high cost existing in identification of Verticillium wilt resistance, fiber quality and yield phenotypic traits in the existing breeding technology, and can simultaneously improve Verticillium wilt resistance , fiber quality and yield traits, improve selection efficiency, and accelerate the breeding process of high-quality, high-yield, disease-resistant new varieties.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular breeding method for synchronously improving cotton verticillium wilt resistance, fiber quality and yield traits. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Upland cotton varieties are planted in a large area in my country. Before the 1980s, cotton breeders mainly focused on improving the yield, but did not pay enough attention to the improvement of fiber quality. As a result, most of the cotton varieties in my country showed high yield and high quality. Difference. Cotton verticil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 石玉真袁友禄李鹏涛李俊文巩万奎龚举武葛群商海红刘爱英王艳玲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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