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Endophytic fungi ES026 of huperzia serrata capable of generating huperzine a

A technology of ES026 and Huperus serrata, which is applied in the field of medicinal plants and agricultural microorganisms, can solve the problems of slow growth, lack of wild resources, and inability to industrialize production, and achieve the effects of protecting the ecological environment, growing rapidly and multiplying, and increasing the yield.

Inactive Publication Date: 2012-09-05
HUAZHONG AGRI UNIV
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Problems solved by technology

[0003] At present, Huperzine A production still relies on extracting from Huperzia serrata plants, which has the following disadvantages: 1. Huperzia serrata belongs to higher ferns, and because of its slow growth and low spore germination rate, wild resources are scarce (Ma X, et al. The Lycopodium alkaloids [J]. Nat. Prod. Rep., 2004, 21: 752-772.)
2. Artificial cultivation technology has not yet been overcome (Li N.Study on the effect of Promote growth on host and fermentation Products of endophytic fungi from Huperzia serrata[D].2007.)
In the 1990s, the upsurge of artificially synthesizing Hup A was set off. Although certain achievements have been made, industrialized production of huperzine A cannot be produced because of its low activity, high cost, and large toxic and side effects (Chen Yegao, etc. Lycopodium alkaloid components New progress in research [J]. Journal of Yunnan Normal University, 2010, 30(6): 12-24.)
However, there is a bottleneck problem in the production of huperzine A in the strains reported at present: low yield

Method used

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  • Endophytic fungi ES026 of huperzia serrata capable of generating huperzine a

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Embodiment 1

[0038] 1. Collect the wild plants of Huperus serrata from Lichuan City, Hubei Province, China, and bring them back to the laboratory for cultivation together with the soil (see figure 1 ).

[0039] 2. Select healthy plants with a plant height of 6-10cm and wash them with running water for 30 minutes to remove surface dirt.

[0040] 3. Surface disinfection, the steps are as follows:

[0041] 1) Soak in 70%-75% alcohol for 2 times, each time for 2-3 minutes.

[0042] 2) followed by 0.1% HgCl 2 Soak for 5 minutes and shake gently 3 times.

[0043]3) Drain the liquid on the plant, then soak it with sterile water for 5 times, and wash it thoroughly to avoid HgCl 2 Residue, and use the last immersion solution to coat on the medium containing PDA (according to the conventional method, ingredients: 200g of potatoes, cut into small pieces, add a small amount of water to boil, glucose 20g, agar 15g / L, pH 6.5 before sterilization; add water to set Make up to 1L, sterilize under hig...

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Abstract

The invention belongs to the technical fields of medicinal plants and agricultural microorganism and particularly relates to an endophytic fungi which is separated from a medicinal plant huperzia serrata, can generate huperzine a or analogues, and can be used in mass production of the huperzine a or analogues. The separated endophytic fungi ES026 is identified as colletotrichum gloeosporioides bymolecular biology and morphology, and the strain has been preserved in China Center For Type Culture Collection (CCTCC), with the preservation number of CCTCC NO: M2011046. The endophytic fungi is significantly characterized in that after liquid fermentation, the strain can generate an active substance with reversibility which can inhibit acetylcholinesterase, and high-performance liquid chromatography and mass spectrum analysis shows that the compound is huperzine a (HupA), thus a new medicinal herb source for treating alzheimer's disease is provided.

Description

technical field [0001] The invention belongs to the technical field of medicinal plants and agricultural microorganisms, and in particular relates to isolating an endophytic fungus from the medicinal plant Huperzia serrata, the bacterial strain is C.gloeosporioides, which can produce huperzine A Or similar, can be used for mass production of huperzine A or similar. Background technique [0002] With the aging of the world's population, Alzheimer's disease (Alzheimers' disease, AD, senile dementia) has become a global public health problem. Alzheimer's disease has a high prevalence rate and a long course of disease, and it is one of the diseases with the highest disability rate among all chronic diseases, which will bring a huge disease burden to both families and society. According to the latest forecast released by the International Alzheimer Federation in 2009, the number of dementia patients worldwide will reach 35.6 million by 2010, 65.7 million in 2030, and 115.4 milli...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/18
Inventor 王沫徐晓苗赵昕梅舒少华徐海杰
Owner HUAZHONG AGRI UNIV
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