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Trichoderma harzianum strain and application thereof

A technology of germs and biological fertilizers, applied in the direction of application, fungicides, fungi, etc., can solve the problems of insufficient follow-up and investigation work, a large amount of manpower labor, and low census efficiency, so as to achieve low cost, promote healthy growth, growth and reproduction fast effect

Active Publication Date: 2017-02-15
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The investigation of rubber tree root disease requires a lot of manpower, the early follow-up investigation work is not in place, and the census efficiency is low
And when obvious symptoms appear in the aboveground part of the rubber tree, it is already the period of severe infection and disease, and the control cost is high and the control effect is poor.

Method used

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  • Trichoderma harzianum strain and application thereof
  • Trichoderma harzianum strain and application thereof
  • Trichoderma harzianum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, screening and identification of Trichoderma harzianum (Trichoderma harzianum) Hb13-3-2 bacterial strain

[0042] 1. Screening of Trichoderma strains

[0043] The Hb13-3-2 strain was isolated from the rhizosphere soil of a rubber tree infected with red root disease in the rubber planting area of ​​Danzhou City, Hainan Province. The soil samples of the 5-10cm soil layer of the rubber tree rhizosphere were collected in order of collection places and separate sequentially numbered. Using the dilution plate method, each soil sample was divided by 10 -3 、10 -4 Diluted at different concentrations, inoculated on Bengal red medium (PDA+chloramphenicol 0.015g / L+streptomycin 0.015g / L+Rose Bengal 0.05g / L), cultured at 28°C for 3 days; picked Trichoderma colony and inoculated in On Bengal red medium (without antibiotics), culture at 28°C for 3 days, further isolate and purify single spores, obtain Trichoderma strains, and then number and identify them.

[0044] The...

Embodiment 2

[0062] The biological characteristic of embodiment 2, Hb13-3-2 bacterial strain

[0063] 1. Carbon source and nitrogen source requirement characteristics of Hb13-3-2 strain

[0064] 1. Determination of the optimum growth medium for Trichoderma harzianum Hb13-3-2 CGMCC No.13163

[0065] Comparison of growth of Trichoderma harzianum (Trichoderma harzianum) Hb13-3-2 CGMCC No.13163 on solid medium (pH6) plates as follows:

[0066] Min(Minimal agar medium) medium: KNO 3 10g, KH 2 PO 4 5g, MgSO 4 ·7H 2 O 0.5g, FeCl 3 0.02g, glucose 10g, agar 20g, dilute to 1000mL;

[0067] PDA (potato dextrose) medium: 200 g of peeled potatoes, 20 g of glucose, 14 g of agar, boil the peeled potatoes for 30 minutes and distill the filtrate to 1000 mL with distilled water;

[0068] CMV (corn flour) medium: corn flour 30g, agar 14g, after boiling for 30min, dilute the filtrate to 1000mL with distilled water;

[0069] OA (oat) medium: oatmeal 30g, agar 14g, after boiling for 30min, dilute th...

Embodiment 3

[0094] Embodiment 3, the tolerance of Trichoderma harzianum (Trichoderma harzianum) Hb13-3-2 bacterial strain to special culture conditions

[0095] 1. Salt-alkali tolerance of strain Hb13-3-2

[0096] Preparation contains salt NaCl (mass percentage concentration is respectively 1%, 3%, 5%, 7%, 10% or 15%) or KCl (mass percentage concentration is respectively 1%, 3%, 5%, 7% , 10% or 15%) PDA medium, PDA medium containing alkali portion KOH (mass percentage concentration is respectively 1%, 3%, 5%, 7% or 9%), containing NaOH (mass percentage concentration 1%, 3%, 5%, 7% or 9%) PDA medium or containing Na 2 CO 3 (1%, 3%, 5%, 7% or 9% by mass percentage concentration respectively) PDA medium, used for detecting the tolerance of Hb13-3-2 bacterial strain to different concentrations of saline-alkali.

[0097] A plate of the above-mentioned medium was prepared and inoculated with Hb13-3-2 strain block, and each medium treatment was repeated three times. Incubate in dark at 28°C ...

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Abstract

The invention discloses a trichoderma harzianum strain and application thereof. The trichoderma strain is a trichoderma harzianum strain Hb13-3-2 CGMCC No. 13163. The strain and metabolites thereof have broad-spectrum activities of inhibiting pathogenic fungi. The contrast growth experiment proves that the trichoderma harzianum strain has the capacity of resisting red root pathogenic bacteria of rubber trees, colletotrichum gloeosporioides and other pathogenic bacteria, and further has the effects of controlling multiple other pollution microbes and promoting growth of rubber seedlings. Therefore, the trichoderma harzianum strain can be immediately applied to the cultivation process of the rubber trees at the seedling stage, healthy growth of the rubber seedlings is promoted, and occurrence of rubber tree root diseases can be shielded or inhibited.

Description

technical field [0001] The invention relates to a strain of multifunctional Trichoderma harzianum strain and application thereof. Background technique [0002] Trichoderma Trichoderma Pers. (the sexual stage was once called "Hypocrea Fr.") belongs to Sordariomycetes, Ascomycota, Hypocreaceae, and Hypocreales. This genus of fungi has a wide variety of species and is widely distributed. It is an important class of renewable natural resources with high economic value and application prospects (Doi 1969, 1972; Zhu Zhaoxiang and Zhuang Wenying, 2014). Trichoderma spp. is an important class of multifunctional probiotic fungi that widely exist in nature. They can not only control the infection of pathogenic bacteria and the occurrence of diseases through a variety of mechanisms, but also have the ability to promote crop growth and improve crop resistance to biotic and abiotic stress. They are recognized as environmentally friendly fungi and are used in agriculture, In the fields ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C05G1/00C05F11/08C12R1/885
CPCA01N63/30C05D9/02C05F11/08C12N1/14C12N1/145C12R2001/885
Inventor 秦云霞阳江华唐朝荣肖小虎龙翔宇方永军戚继艳
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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