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Bacillus licheniformis 201 for highly effective preventing and curing banana wilt and application thereof

A technology of Bacillus licheniformis and banana wilt bacteria, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of blank and weak technology, and achieve the effects of easy storage, good development and application prospects, and simple cultivation conditions

Inactive Publication Date: 2008-05-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Isolation and screening from banana plants to obtain biocontrol bacterial strains with antagonistic effects on Fusarium wilt of banana is of great significance to the control of Fusarium wilt of banana, but the relevant technology is still weak or even blank

Method used

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  • Bacillus licheniformis 201 for highly effective preventing and curing banana wilt and application thereof
  • Bacillus licheniformis 201 for highly effective preventing and curing banana wilt and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Inhibitory effect of Bacillus licheniformis 201 on mycelia FOC4 of Fusarium wilt fungus No. 4 race

[0021] Insert the 5mm diameter mycelium block of mycelium FOC4 of the banana fusarium wilt fungus race 4 into the center of the PDA plate of potato sucrose medium, and inoculate three pieces of sterilized filter paper with a diameter of 5mm around the FOC4 mycelium block at equal distances. 10 μL of culture solution of strain 201 cultured for 24 h was added dropwise on the filter paper sheet. Repeat three times, culture at 25-28°C, and measure the diameter of the inhibition zone after 5 days. It can be seen from accompanying drawing 1 that bacillus licheniformis 201 has a strong bacteriostatic effect on banana wilt.

Embodiment 2

[0022] Example 2 Inhibition of Bacillus licheniformis 201 on Banana Fusarium Wilt under Greenhouse Conditions

[0023] The Brazilian banana seedlings with a plant height of about 10 cm were immersed in the culture solution of the strain 201, and the OD of the culture solution was equal to 0.5. After 30 minutes, they were taken out and planted in flower pots. On the 2d, 7d, 15d and 30d respectively, pick up the banana seedlings, immerse them in the spore suspension of FOC4 for 30min, the spore suspension is 1×105 spores / mL, and then plant them in flower pots. Treat 36 plants in pots. After inoculation, the banana seedlings were placed in a greenhouse at 23-25°C for cultivation, and water was poured once per eye. The incidence rate and disease index were investigated 20 days, 30 days and 40 days after FOC4 inoculation. The results showed that the challenge inoculation with FOC4 after strain 201 was inoculated 15 days and 30 days later, compared with the control, the incidence ...

Embodiment 3

[0024] Example 3 Teratogenic effect of Bacillus licheniformis 201 on FOC4 hyphae

[0025] Cut the cellophane into strips of 5mm×25mm, autoclave at 121°C, and place it between the strain 201 and the pathogen FOC4 with tweezers. Cultivate at a constant temperature of 28°C for 5 to 7 days. After an obvious bacteriostatic zone appears between the mycelium of strain 201 and FOC4, carefully remove the cellophane and observe the morphological changes of the mycelium under a microscope with a power of 10×40. The pathogenic bacteria of strain 201 was used as a control. It was observed that the hyphae were enlarged and prominent in the early stage, and obviously thinned in the later stage, with many and short branches and uneven shape, see Figure 2, in which 1: normal growth of FOC4 hyphae (10×40 times); 2: hyphae swelling in the early stage Prominent (10×40 times); 3: Most of the hyphae become tapered in the later stage, with many and short branches, and uneven shape (10×40 times).

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Abstract

The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to an endogenous biocontrol bacterial strain for preventing and treating banana wilt, in particular to bacillus licheniformis 201 for efficiently preventing and controlling banana wilt and its application. Background technique [0002] Banana Fusarium wilt is a devastating disease caused by the infection of Fusarium oxysporum f.sp.cubense, and it is a devastating soil-borne disease of banana production. The pathogen invades from the injured rhizome, spreads to the pseudostem and leaves through the vascular bundle, and blocks the xylem vessel until the plant withers and dies. Fusarium wilt of banana is a soil-inhabiting fungus, and its chlamydospores can survive in the soil for 8 to 10 years. Banana Fusarium wilt is distributed in many counties and cities in the banana production area of ​​the Pearl River Delta in China. The rate of diseased plants in the diseased garden is about 10%...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/10
Inventor 王振中王春华纪春艳孙正祥
Owner SOUTH CHINA AGRI UNIV
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