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Bacillus velezensis having various disease preventive effects, application thereof and biocontrol inoculant

A technology of bacillus and biocontrol agent, applied in the direction of application, biocide, fungicide, etc.

Active Publication Date: 2019-08-16
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports about the antagonistic control effect of Bacillus Velez on fruit trees and other diseases at the same time.

Method used

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  • Bacillus velezensis having various disease preventive effects, application thereof and biocontrol inoculant
  • Bacillus velezensis having various disease preventive effects, application thereof and biocontrol inoculant
  • Bacillus velezensis having various disease preventive effects, application thereof and biocontrol inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In this example, the Bacillus velezensis with multiple disease prevention functions is Bacillus velezensis MS012, and the preservation number is CCTCC NO: M 2019267; preservation date: April 17, 2019, Preservation Unit: China Type Culture Collection Center, Preservation Address: Wuhan, China. Wuhan University (Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province).

[0033] Example 1 of the application of Bacillus velei with multiple disease-preventing effects in antagonizing plant pathogenic bacteria

[0034] The bacterial strain used in this example is Bacillus velezensis MS012, and the preservation number is CCTCC NO: M 2019267. The phytopathogens are Botrytis botrytis, Glososporum anthracnose, and / or Phomopsis appleus.

Embodiment 2

[0036] The bacterial strain used in this example is Bacillus velezensis MS012, and the preservation number is CCTCC NO: M 2019267. The plant pathogenic bacteria are wheat sheath blight, wheat take-all, tomato cinerea, wheat scab, Clivia stem rot, and / or Nandina anthracnose.

Embodiment 3

[0038] The bacterial strain used in this example is Bacillus velezensis MS012, and the preservation number is CCTCC NO: M 2019267. The plant pathogen Erwinia amylovora.

[0039] Embodiment 1 of the biocontrol bacterium agent with various disease prevention effects

[0040] The biocontrol bacterium agent with multiple disease prevention functions in this example includes biocontrol bacteria and auxiliary materials. The biocontrol bacterium is Bacillus velezensis MS012, and the preservation number is CCTCC NO: M 2019267. Adjuvants can be those commonly used in this field.

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Abstract

The invention relates to bacillus velezensis having various disease preventive effects, application thereof and a biocontrol inoculant and belongs to the technical field of plant disease and pest prevention and control. Bacillus velezensis MS012 having bacteriostatic activity is obtained by separating and screening from healthy branches of malus sieversii. Bacillus amyloliquefaciens has remarkableantagonistic effect on botryosphaeria dothidea, colletotrichum gloeosporioides, phomopsis mali, rhizoctonia cerealis, gaeumannomyces graminis, botrytis cinerea, fusarium graminerum, clivia stem basaldecay pathogen, nandina anthrax pathogen and erwinia amylovora and has broad spectra in bacteriostatic object, and a new microbial resource is provided for biocontrol on disease and pest of plants like apple trees.

Description

technical field [0001] The invention relates to Bacillus velei with multiple disease-preventing functions, its application and a biocontrol agent, and belongs to the technical field of plant disease prevention and control. Background technique [0002] At present, the applied biocontrol bacteria mainly include Bacillus spp., Pseudomonas spp., Streptomyces spp. and some other beneficial bacteria, among which Bacillus is the most widely used. Bacillus widely exists in air, water, soil and other environments, and is widely used in the biological control of plant diseases, and plays its role in biocontrol by producing antibacterial proteins or antimicrobial peptides. For example, Bacillus subtilis (Bacillus subtilis) F3 can produce an antibacterial protein (ZhaoXY, Zhao XM, Wei YM, Shang QX, Liu ZP. isolation and identification of a novelantifungal protein from a rhizobacterium Bacillus subtilis strain F3.Journalof Phytopathology, 2012,161(1):43-48.); Bacillus cereus (Bacillus ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 李永丽周洲曲良建常乐史娟余海如
Owner HENAN UNIV OF SCI & TECH
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