Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof

A technology of Bacillus subtilis and probiotics, applied in the field of probiotics, can solve the problem of few Bacillus subtilis fermented and cultivated by Astragalus membranaceus, and achieve the effects of improving synergistic effect, easy operation, and improving animal production performance

Active Publication Date: 2017-04-26
沈阳丰美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, there are many studies on the fermentation of Astragalus membranaceus (solid and liquid fermentation), astragalus medicinal residues and astragalus extracts by lactic acid bacteria, but there are relatively few related reports on the fermentation of Astragalus membranaceus to cultivate Bacillus subtilis

Method used

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  • Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof
  • Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof
  • Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof

Examples

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Effect test

Embodiment 1

[0032] A micro-ecological preparation of astragalus fermented by Bacillus subtilis, said micro-ecological preparation is prepared by fermenting a strain of Bacillus subtilis FM537 on a fermentation substrate containing astragalus water extract, wherein the fermentation substrate is: tryptone 15g / L, glucose 15g / L, beef extract 50g / L, ammonium sulfate 0.5g / L, sodium chloride 1g / L, sodium dihydrogen phosphate 0.5g / L, distilled water, adjust the pH to 7.2, and then according to the volume ratio Add 45-50% of astragalus water extract and sterilize under high pressure; the preservation number of the bacillus subtilis FM537 is CGMCC No.12848.

[0033] The Bacillus subtilis is inoculated into the fermentation substrate in the form of a secondary seed liquid. The preparation method is: streak the Bacillus subtilis FM537 on an LB agar plate, cultivate overnight at 37° C., pick a single colony and inoculate it in 10 mL of LB liquid medium, 150rpm, 37°C overnight culture as the primary se...

Embodiment 2

[0046] The application effect of the microecological preparation of astragalus fermented by Bacillus subtilis in the feeding of laying hens.

[0047] A total of 1440 Hai-Line brown layer hens with similar body weight and laying rate at 300 days were selected and randomly divided into 3 treatments, each treatment had 10 replicates, and each replicate had 38 chickens. Treatment group 1 is a control group without adding the probiotics of the present invention; treatment groups 2 and 3 are test groups, respectively adding 0.5% and 1% of the probiotics of Example 1 of the present invention to their drinking water. The same diet was fed, and the test period was 70 days. After the test, the average feed intake, egg production rate, feed-to-egg ratio, egg production and other indicators were calculated, and 30 eggs were randomly picked from each treatment group, and the eggs were tested. Shell strength, eggshell thickness, Hough units, yolk color and egg shape index. The basic diet f...

Embodiment 3

[0059] Effect of the microecological preparation of astragalus fermented by Bacillus subtilis of the present invention on the production performance of broiler chickens.

[0060] A total of 216 1-day-old healthy AA broilers were selected and randomly divided into 3 treatments, each treatment had 6 replicates, and each replicate had 12 chickens. Treatment group 1 is a control group without adding the probiotics of the present invention; treatment groups 2 and 3 are test groups, respectively adding 0.5% and 1% of the probiotics of Example 1 of the present invention to drinking water. Feed the same diet, the test period was 20 days, at the end of the test, calculate the average daily feed intake, average daily gain, feed-to-meat ratio and mortality rate. Blood was collected from broiler chickens the next morning after the end of the experiment, and serum was prepared and stored in a -20°C refrigerator. IgG and IgM in the serum of each test group were detected using kits and micro...

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Abstract

The invention provides a microecological preparation with bacillus subtilis fermenting astragalus membranaceus and an application thereof, wherein the microecological preparation is prepared through fermentation by means of a bacillus subtilis strain FM537 on a fermentation substrate containing astragalus membranaceus water extract liquid. The fermentation substrate comprises 15 g/L of tryptone, 15 g/L of glucose, 50 g/L of beef extract, 0.5 g/L of ammonium sulfate, 1 g/L of sodium chloride and 0.5 g/L of sodium dihydrogen phosphate. The fermentation substrate is prepared with distilled water, wherein pH is regulated to 7.2, and then according to volume ratio, 45-50% of the astragalus membranaceus water extract liquid is added, and finally high-pressure sterilization is carried out to produce the fermentation substrate. Through ultrasonic wave for assisting water extraction of the astragalus membranaceus, the extraction time is greatly reduced. Through selection of suitable fermentation temperature and fermentation time, synergistic effect of the astragalus membranaceus and the bacillus subtilis is improved. The microecological preparation is used as a livestock and poultry feed additive, and can improve animal production performance, digestion rate and body immunity.

Description

technical field [0001] The invention relates to a microecological preparation of astragalus fermented by Bacillus subtilis and an application thereof, belonging to the technical field of biological fermentation of traditional Chinese medicines. Background technique [0002] In recent years, the negative effects brought about by the widespread application of antibiotics and chemical antibacterial drugs in the livestock and poultry industry have attracted increasing attention. For example, antibiotics lead to the emergence of a large number of drug-resistant strains, resulting in the imbalance of normal flora in animals; at the same time, the toxic and side effects brought about by drug residues are seriously endangering the health of animals and humans, and reducing the value of livestock and poultry products in the international market. Therefore, many countries have successively restricted or banned the application of certain antibiotics in animal production. In 2006, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/18A23K10/30C12R1/125
CPCC12N1/20
Inventor 王丽娟郑遂丁毅杨晓波张鑫
Owner 沈阳丰美生物技术有限公司
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