Household harmful microorganism elimination and deodorization solution and preparation method thereof
A technology of deodorant liquid and probiotic liquid, which is applied in the field of daily necessities, can solve the problems of inhibiting harmful bacteria and deodorizing effect not enough and fast, unable to change the formation of odor or prevent its emission, and has not been popularized and applied. Achieve the effects of no irritating odor, low cost, and no more odor
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Embodiment 1
[0041] (1) Activation and multiplication:
[0042] A. The cultivation of photosynthetic bacteria: inoculate the original bacteria of green sulfur bacteria into a liquid test tube with a cover, and cultivate under light at 30°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into an Erlenmeyer flask. The culture medium is a modified paradigm culture medium (sterilized at 121°C for 30 minutes), and then the secondary culture is shaken at a temperature of 30°C and 120 r / min. After 48-72 hours of cultivation, the secondary culture of Rhodobacter bacillus is obtained.
[0043] Cultivation of Bacillus: put the original strain of Bacillus subtilis on the nutrient agar medium, and cultivate it on a primary slant at 36°C for 24 hours, then inoculate it into the nutrient broth medium for shaking secondary cultivation, the temperature is 36°C, 120r / min, after culturing for 48-72 hours, the secondary culture of Bacillus was obtained.
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Embodiment 2
[0058] (1) Activation and multiplication
[0059] A. Cultivation of photosynthetic bacteria: inoculate the original bacteria of red bacillus into a liquid test tube with a cover, and cultivate under light at 31°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a conical flask. The culture medium is an improved culture medium (sterilized at 121°C for 30 minutes), followed by shaking secondary culture at a temperature of 31°C and 120 r / min. After 48-72 hours of cultivation, a secondary culture of Rhodobacter bacilli was obtained.
[0060] B. Bacillus culture: the original strain of Bacillus cereus was placed on beef extract peptone medium, and cultured on a slant for the first level at 32°C, and then inoculated into the same liquid corn steep liquor medium for shaking secondary culture, at a temperature of 32°C. 120r / min, after culturing for 48-72 hours, a secondary culture of Bacillus cereus was obtained.
[0061] C Cultiva...
Embodiment 3
[0076] (1) Activation and multiplication
[0077] A. The cultivation of photosynthetic bacteria: inoculate the original purple non-sulfur bacteria into a liquid test tube with a cover, and cultivate under light at 31°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a conical flask. The culture medium is a modified paradigm culture medium (sterilized at 121°C for 30 minutes), and then the secondary culture is shaken at a temperature of 31°C and 120r / min. After 48-72 hours of cultivation, a secondary culture of purple non-sulfur bacteria is obtained.
[0078] Cultivation of Bacillus: put the original strain of Bacillus licheniformis on the wort medium, do primary slope culture at 31°C, then inoculate into the same liquid wort medium for shaking secondary cultivation, temperature 31°C, 120r / min, cultivated for 48-72 hours to obtain a secondary culture of Bacillus licheniformis.
[0079] C Cultivation of lactic acid bacteria:...
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