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Method for manually inducing Aquilaria sinensis to generate agilawood

A technology of artificial induction and white woody fragrance, which is applied in the direction of botany equipment and methods, chemicals for biological control, biocides, etc., can solve the constraints of sustainable development and utilization of agarwood, inconspicuous effect of agarwood, long growth cycle, etc. problems, to achieve the effect of meeting market demand, low cost, and easy operation

Active Publication Date: 2012-01-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2005, Subehan found that Fusarium laseritum can quickly infect agarwood trees through a survey of Indonesian agarwood trees. This kind of fusarium is easy to isolate and cultivate, and drill holes on the trunk for inoculation. Agarwood can be obtained after one year, but the quality is relatively low. Difference
Agarwood is a perennial tree with a long growth cycle, and it does not produce fragrance without any damage or stimulation. Using ordinary methods such as manual cutting, the effect of producing fragrance is not obvious, which seriously restricts the sustainable development of agarwood. use

Method used

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  • Method for manually inducing Aquilaria sinensis to generate agilawood
  • Method for manually inducing Aquilaria sinensis to generate agilawood
  • Method for manually inducing Aquilaria sinensis to generate agilawood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Such as figure 1 and figure 2 As shown, the infusion device 1 of this embodiment includes an infusion bottle 11 , a connecting tube 12 connected to the opening of the infusion bottle, and the other end of the connecting tube is connected with an infusion head 13 . Select the 3-year-old white wood fragrant tree 2, use a drill bit to drill holes in the trunk, the holes are slightly inclined downwards, and the depth reaches 2 / 3 of the diameter of the trunk, and the hole spacing is 50cm. Such as figure 2 The infusion device is installed as shown, the infusion bottle 1 is suspended on the Arboria japonica tree 2, and the infusion head 13 is inserted into the hole. Then drop formic acid, acetic acid and sodium dihydrogen phosphate solution (solvent is water) of different pH values ​​on different Arakita japonica trees, each 500ml of sterile water, after 12 months, cut down the Acacia radiata trees, cut them longitudinally, Observe the formation of agarwood, and the speci...

Embodiment 2

[0027] Choose the 5-year-old white wood fragrant tree, utilize a drill bit to drill a hole on the trunk, the drill hole is slightly downwardly inclined, and reach 2 / 3rds of the trunk diameter deeply, and the hole spacing is 150cm. The dripping device is installed according to the method of embodiment 1, and the dripping device is dripped. Add solution. At first each treatment drops 250ml of formic acid solution of pH value 2.0 first, then drips respectively 250ml of different types of endophytic fungus culture solution obtained by separating from A. Gauze filters out most of the mycelia, so that only conidia and a small amount of mycelia are contained in the nutrient solution, for subsequent use, the fungus liquid of the following examples is the same as the cultivation method here), and after adding 250ml of formic acid with a pH value of 2.0, drop Add 250ml of sterile water or drop 250ml of formic acid with a pH value of 2.0 first and then do not do any treatment as a contro...

Embodiment 3

[0034] Choose the 10-year-old white wood fragrant tree, use a drill to drill two holes on the trunk, the holes are slightly inclined downwards, and reach two-thirds of the trunk diameter deeply, the hole spacing is 120-150cm, and the drip is installed according to the method of embodiment 1 Inject the device and add the solution dropwise. First, 500ml of formic acid with a pH value of 2.0 was added dropwise, and then 500ml of culture solution of the fungus Botryosphaeria rhodina was added dropwise. After 12 months, the tree was cut down, cut open longitudinally, and the formation of agarwood was observed. The specific results are shown in Table 4.

[0035] Table 4

[0036]

[0037] The length of the agarwood resin produced by dripping reaches 3.8 meters, the widest part of the longitudinal section reaches 6cm, and the discoloration area is relatively large, which is estimated to reach 1500cm 2 Above, the fragrant parts of some white wood incense trees can extend into their...

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Abstract

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for artificially inducing agarwood to produce agarwood. Background technique: [0002] Agarwood (Aquilaria Resin, Aloeswood, Agarwood or Eaglewood) is a traditional precious medicine with a long history of application, as well as precious natural spices and wood. Agarwood has a long history of being used as medicine, with a history of at least 3,000 years in China and Japan. "Compendium of Materia Medica" records that agarwood can "cure upper heat and lower cold, reverse qi and dyspnea, deficiency and closure of the large intestine, urination, urination, and men's coldness". Agarwood is pungent in nature, slightly warm, non-toxic, has the effects of promoting qi and analgesia, warming the middle to stop vomiting, inhaling qi and relieving asthma, etc. It has obvious curative effects on treating abdominal distension, cold stomach, kidney deficiency, and asthma. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N59/26A01N37/02A01P21/00
Inventor 王磊章卫民高晓霞吴关庆李浩华陈庆
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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