Biomass charcoal based probiotic preparation and preparation method thereof
A probiotic and probiotic technology, applied in the field of probiotics, can solve problems such as water pollution and lake eutrophication, and achieve the effects of strong fertilizer absorption, energy saving in the production process, and good slow release effect.
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Embodiment 1
[0023] 1. Strain selection: the selected strains include: Paenibacillus polymyxa and Trichoderma, with a weight ratio of 1:1.
[0024] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 3ml of the activated strains are inserted into a triangular flask equipped with 250ml of medium for liquid shaker culture, and then the obtained 700kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 15L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 2kg of probiotics.
[0025] 3. Preparation of biomass carbon black: use local agricultural waste stalks to carbonize at low temperature into 18kg of porous biomass carbon black.
[0026] 4. Preparation of biochar-based probiotic agent: Take 18kg of biomass carbon black and 2kg of probiotic agent, and carry out mixed adsorption to form 20kg ...
Embodiment 2
[0028] 1. Strain selection: the selected strains include: Paenibacillus polymyxa, Potassium bacteria and Bacillus subtilis, the weight ratio of which is 1:1:2.
[0029] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 5ml of the activated strains are inserted into a triangular flask equipped with 500ml of medium for liquid shaker culture, and then the obtained 800kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 20L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 1.58kg of probiotics.
[0030] 3. Preparation of biomass carbon black: use local biomass raw materials such as salix stubble, and carbonize at low temperature into 17kg of porous biomass carbon black.
[0031] 4. Preparation of biochar-based probiotics: Take 17kg of biomass carbon black and 1.58kg of probio...
Embodiment 3
[0033] 1. 1. Strain selection: the selected strains include: phosphate solubilizing bacteria, potassium solubilizing bacteria and cellulolytic bacteria, the weight ratio of which is 1:1:1.
[0034] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 5ml of the activated strains are inserted into a triangular flask equipped with 500ml of medium for liquid shaker culture, and then the obtained 750kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 20L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 1.95kg of probiotics.
[0035] 3. Preparation of biomass carbon black: use local biomass raw materials such as salix stubble, and carbonize at low temperature into 15kg of porous biomass carbon black.
[0036] 4. Preparation of biochar-based probiotics: Take 15kg of biomass carbon...
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