Biomass charcoal based probiotic preparation and preparation method thereof

A probiotic and probiotic technology, applied in the field of probiotics, can solve problems such as water pollution and lake eutrophication, and achieve the effects of strong fertilizer absorption, energy saving in the production process, and good slow release effect.

Inactive Publication Date: 2012-10-03
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to surveys, the utilization rate of chemical fertilizers in my country is only 35%-40%, and the rest is fixed by the soil, or leached to cause environmental problems such as water pollution and eutrophication of lakes.
In addition, chemical fertilizer is a high-energy-consuming product. Due to the impact of rising raw material prices and environmental pollution, many small chemical fertilizer plants are on the verge of closing down. However, due to economic and energy constraints, the country is currently unable to transform these chemical fertilizer plants. The increase in the total amount of fertilizer in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Strain selection: the selected strains include: Paenibacillus polymyxa and Trichoderma, with a weight ratio of 1:1.

[0024] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 3ml of the activated strains are inserted into a triangular flask equipped with 250ml of medium for liquid shaker culture, and then the obtained 700kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 15L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 2kg of probiotics.

[0025] 3. Preparation of biomass carbon black: use local agricultural waste stalks to carbonize at low temperature into 18kg of porous biomass carbon black.

[0026] 4. Preparation of biochar-based probiotic agent: Take 18kg of biomass carbon black and 2kg of probiotic agent, and carry out mixed adsorption to form 20kg ...

Embodiment 2

[0028] 1. Strain selection: the selected strains include: Paenibacillus polymyxa, Potassium bacteria and Bacillus subtilis, the weight ratio of which is 1:1:2.

[0029] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 5ml of the activated strains are inserted into a triangular flask equipped with 500ml of medium for liquid shaker culture, and then the obtained 800kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 20L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 1.58kg of probiotics.

[0030] 3. Preparation of biomass carbon black: use local biomass raw materials such as salix stubble, and carbonize at low temperature into 17kg of porous biomass carbon black.

[0031] 4. Preparation of biochar-based probiotics: Take 17kg of biomass carbon black and 1.58kg of probio...

Embodiment 3

[0033] 1. 1. Strain selection: the selected strains include: phosphate solubilizing bacteria, potassium solubilizing bacteria and cellulolytic bacteria, the weight ratio of which is 1:1:1.

[0034] 2. Expansion and cultivation into probiotics: After the strains that have been screened are inserted into the liquid medium for activation, 5ml of the activated strains are inserted into a triangular flask equipped with 500ml of medium for liquid shaker culture, and then the obtained 750kg of fermentation liquid was obtained through step-by-step expansion and cultivation of 20L and 1t seed tanks. The solid-liquid separation was carried out by a disc centrifuge, and then flash-dried by a flash dryer to obtain 1.95kg of probiotics.

[0035] 3. Preparation of biomass carbon black: use local biomass raw materials such as salix stubble, and carbonize at low temperature into 15kg of porous biomass carbon black.

[0036] 4. Preparation of biochar-based probiotics: Take 15kg of biomass carbon...

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PUM

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Abstract

The invention discloses a biomass charcoal based probiotic preparation, which is composed of biomass carbon black and concentrated probiotic preparation based on a weight ratio of (8.85-9.15): (0.85-1.15). The preparation is prepared by using agricultural wastes as biomass raw materials, conducting low-temperature carbonization to form porous biomass carbon black; conducting amplification culture to beneficial microorganism, and fermenting to produce probiotic solution; mixing above materials and separating solid from liquid; and drying by flash evaporation to obtain concentrated probiotic preparation, wherein the beneficial microorganism is one or more of paenibacillus polymyxa, trichoderma, nitrogen-fixing bacteria, rhizobium, phosphate dissolving bacteria, potassium dissolving bacteria, cellulose decomposition bacteria, antibiotic producing bacteria, and photosynthetic bacteria. In addition, the invention also discloses a preparation method for the biomass charcoal based probiotic preparation. The biomass charcoal based probiotic preparation provided by the invention uses microporous biomass charcoal, which has very strong absorption capacity and very good light absorption and warming effect. The probiotic preparation is high in survival rate of live bacteria, can be preserved for a long time at normal temperature and has evident synegistic effect for fertilizer.

Description

technical field [0001] The invention relates to a probiotic agent, in particular to a biochar-based probiotic agent; in addition, the invention also relates to a preparation method of the biochar-based probiotic agent. Background technique [0002] my country is a country with a large population and a large agriculture. It uses 7% of the world's land to feed 22% of the world's population. The food issue is always a major issue related to the national economy and people's livelihood. my country has 2 billion mu of arable land and needs 150 million tons of fertilizers annually. It is the world's largest importer of fertilizers and producer of nitrogen fertilizers. Even so, the amount of chemical fertilizers still cannot meet the needs of agricultural development. The reason is that the key problem is that the utilization rate of chemical fertilizers is too low. According to surveys, the utilization rate of chemical fertilizers in my country is only 35%-40%, and the rest is f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/08C05G3/80C05G3/90
CPCY02P60/21Y02A40/22
Inventor 吴奇能王奕然李玉梅潘文灏
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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