Bactericidal composition containing benziothiazolinone and methoxyl acrylic ester type bactericides
A methoxyacrylic acid and composition technology, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of easy generation of drug resistance, single action site, etc., to reduce the dosage, reduce pollution, and delay resistance. medicinal effect
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[0017] Bioassay example 1
[0018] Pot-cultivation test of combination of thiophene ketone and fenoxystrobin on cucumber downy mildew
[0019] Test object: cucumber downy mildew (pseudoperonospora cubensis) collected from the field
[0020] Test method: Refer to "People's Republic of China Agricultural Industry Standard NY / T1156.11-2008". In the pot method, two cucumber seedlings at the true leaf stage with basically the same growth were selected as experimental materials, and 5 pots of cucumber seedlings were selected for each treatment, numbered for testing. Taken from the cucumber downy mildew leaves in the field, wash the pathogen with sterile water with a writing brush to prepare a spore bunch suspension with a concentration of 3×105 spore bunches / ml, and spray inoculate it on cucumber fungus leaves (10 ml per treatment ). After inoculation, moisturizing culture, treatment with medicine 24h later. See Table 1 for test agents. Use Potter spray tower to spray at 50PSI pressur...
Example Embodiment
[0031] Bioassay example 2
[0032] Indoor virulence determination of thiophene ketone and kresoxim-methyl on the pathogen of wheat powdery mildew
[0033] Test object: Wheat powdery mildew collected from the field
[0034] Test method: Refer to "The People's Republic of China Agricultural Industry Standard NY / T1154.7-2006". The powdery mildew diseased leaves of wheat collected from the field were inoculated with conidia evenly shaking off the wheat seedlings. After 24 hours, the agent was treated. The test agents and the virulence test results are shown in Table 2. The calculation formulas for ATI, TTI and CTC are the same as in Bioassay Example 1.
Example Embodiment
[0035] Bioassay example 3
[0036] Indoor Toxicity Determination of Combination of Thiamphenone and Azoxystrobin to Cucumber Botrytis cinerea
[0037] Test object: Cucumber Botrytis cinerea collected from the field
[0038] Test method: Refer to "The People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", mycelial growth rate method. Cucumber Botrytis cinerea was cultured with PDA medium, and when the colony just overgrown the petri dish, punched holes from the edge with a punch with an inner diameter of 7mm, and the punched hyphae block was used as an inoculum. Take 5ml of the prepared medicinal solution and 75ml of sterile culture medium and mix them evenly to make 4 medicine-containing plates. Mix the same amount of sterile water with the culture medium as a control. Place the mycelium block upside down and connect it to the center of the plate, and culture it in a 28°C incubator. After 7 days, the colony diameter was measured by the cross method, and the ne...
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