Streptomyces alboflavus, method using Streptomyces alboflavus to prepare biocontrol microbial agent and application

A technology of Streptomyces alba and biocontrol microorganisms, which is applied in the field of agricultural microorganisms and achieves the effects of short fermentation culture period, good application prospect and simple preparation process.

Active Publication Date: 2019-01-04
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biological control of Ralstonia solanacearum mainly focuses on the use of bacteria such as Bacillus and Pseudomonas, and there are few reports on the use of actinomycetes, especially Streptomyces, to inhibit Ralstonia solanacearum.

Method used

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  • Streptomyces alboflavus, method using Streptomyces alboflavus to prepare biocontrol microbial agent and application
  • Streptomyces alboflavus, method using Streptomyces alboflavus to prepare biocontrol microbial agent and application
  • Streptomyces alboflavus, method using Streptomyces alboflavus to prepare biocontrol microbial agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0046] (1) Preparation of culture medium

[0047] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0048] Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; tap water 1000mL, pH7.2.

[0049] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL. The concentration of other inorganic salts with different concentrations is adjusted on this basis.

[0050] Fermentation medium: Low-value grains soaked in tap water for 6-8 hours (grai...

Embodiment 2

[0064] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0065] (1) Preparation of culture medium

[0066] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0067] Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; tap water 1000mL, pH7.2.

[0068] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL.

[0069] Fermentation medium: Low-value grains soaked in tap water for 6-8 hours (grains such as old rice, old corn, old sorghum, and old wheat that have been stored for more than one yea...

Embodiment 3

[0083] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0084] (1) Preparation of culture medium

[0085] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0086]Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; Tap water 1000mL, pH7.2.

[0087] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL.

[0088] Fermentation medium: low-value grains soaked in tap water for 6-8 hours (grains such as old rice, old corn, old sorghum, and old wheat that have been stored for more than a year),...

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Abstract

The invention relates to Streptomyces alboflavus, a method using the Streptomyces alboflavus to prepare a biocontrol microbial agent and application. The method includes: placing the Streptomyces alboflavus into a preservation culture medium, activating at 28-32 DEG C for 5-7 days, placing into a seed culture medium, and culturing at 30 DEG C and 160-180r / min for 36-48 hours to obtain a seed bacterium solution; inoculating the seed bacterium solution into a fermentation culture medium, performing fermentation culture in a constant-temperature culture tank of 30 DEG C for 7 days, placing the matrix obtained after the fermentation culture into a ventilation drying oven of 25-35 DEG C or an air energy heat pump drying machine for drying or subjecting the matrix to natural drying under normaltemperature, and crushing to obtain the biocontrol microbial agent. The prepared biocontrol microbial agent is applicable to ralatonia solanacearum antagonism, applicable to salt resistance, drought resistance and soil inorganic phosphorus dissolving, applicable to siderophore and growth hormone heteroauxin synthesis, and applicable to tomato seed germination promotion and growth promotion.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, in particular to Streptomyces alboflavinum and a method and application of using the microorganism to prepare a biocontrol microbial agent. Background technique [0002] With the improvement of people's living standards, especially people's requirements for improved quality of life, the world is actively developing green and organic ecological agriculture. In the process, it is required to use no or limited use of chemical fertilizers, chemical pesticides and other chemical substances, etc. . However, at present, most of the prevention and control of plant diseases still rely on chemical agents for prevention and control, which has an impact on the ecological environment. The resistance of pathogenic microorganisms is increasing, and it also seriously affects the quality of agricultural products and the health of the people, which is not conducive to national health and our co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P21/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 王昌禄薛意斌路来风李贞景刘春静武淑芬陈勉华郭庆彬刘欢欢杨明冠李风娟
Owner TIANJIN UNIV OF SCI & TECH
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