A Trichoderma aculeatus strain Sanju-19 and its application
A technology of Trichoderma aculeatus and Trichoderma, which is applied in the field of bioengineering, can solve the problems of intolerant storage, unsightly appearance of diseased potatoes, and small damage area.
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[0016] 1. Acquisition, isolation and identification of strains
[0017] The strains were isolated and identified by collecting soil samples from banana diseased soil in Fujian.
[0018] Colony morphology: grows fast in wort juice medium, cultivated in the dark at 25°C for 5 days, the colony diameter is 60-65mm, white, and in the later stage due to sporulation, it is light gray green and flocculent; the back is light brown, without water-soluble pigment. A large number of conidia were produced, and the sporulation area was evenly distributed. Conidiophores are tall, with smooth or slightly rough walls, usually branched at opposite positions, 2.5-5.0um wide; few phialides, several clustered on spores or their branches, with a curved flask-shaped neck, 5.0-16.7*2.5-3.5um; conidia are broadly oval or nearly spherical, with smooth or slightly rough walls, 2.1-4.5um; chlamydospores are more common.
[0019] Molecular identification method: ITS1 / 5.8SrRNA, ITS2 full sequence and 28S...
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