Bacillus siamensis B-3 and application thereof

A technology of bacillus and bacterial agent, applied in the biological field, can solve the problems of environmental pollution, decreased control effect, endangering the health of humans and animals, etc.

Active Publication Date: 2017-08-11
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of chemical agents, on the one hand, pathogenic bacteria are likely to develop drug resistance, thereby greatly reducing the control effect; on the other hand, it is easy to cause serious environmental pollution, endangering the health of humans and animals

Method used

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  • Bacillus siamensis B-3 and application thereof
  • Bacillus siamensis B-3 and application thereof
  • Bacillus siamensis B-3 and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0057] The preparation method of potato liquid medium is as follows: take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of distilled water, and boil for 30 minutes; collect the filtrate after gauze filtration, add 20.0 g of glucose to the filtrate, and distill the volume to 1.0 L, the pH value is natural, and then sterilized at 115°C for 15 minutes.

[0058] The preparation method of potato liquid culture medium 1 is: take 200g of peeled potatoes, cut into small pieces, add 1.0L distilled water, boil for 30min; collect the filtrate after gauze filtration, add glucose 20.0g in the filtrate, and distilled water to volume 1.0L, adjust the pH to 4.0; then sterilize at 115°C for 15 minutes.

[0059] Potato liquid medium 2: In the preparation method of potato liquid medium 1, "adjust the pH value to 4.0" was replaced with "adjust the pH value to 5.0", and the other steps were the same.

[0060] Potato liquid medium 3: In the preparation method of potato liquid med...

Embodiment 1

[0065] Example 1. Isolation, Identification and Preservation of Bacillus siamensis B-3 CGMCC No.13753

[0066] 1. Separation

[0067] 1. Processing of soil samples

[0068] (1) Add 10 g of soil sample (collected from the potato soil in Pingjibao, Ningxia Hui Autonomous Region) and an appropriate amount of glass beads to 90 mL of sterile water, shake for 10 min, and let it stand for 30 s to make a soil stock solution (at this time, the dilution is recorded as 10 -1 ). Take 1 mL of the supernatant of the soil stock solution and add it to a test tube filled with 9 mL of sterile water and mix well (the dilution is 10 at this time). -2 ), then draw 1mL from this test tube and add it to another test tube filled with 9mL sterile water and mix well, and so on to make dilution A (dilution is 10 -3 ) and diluent B (dilution of 10 -4 ).

[0069] 2. Take Streptomyces scab 4.76, inoculate it on an oat solid plate, and culture it statically at 28°C for 10 days; then add an appropriate ...

Embodiment 2

[0082] The propagation of embodiment 2, bacillus siamese B-3

[0083] The specific steps of the Bacillus siamese B-3 propagation of embodiment 1 are as follows:

[0084] 1. Activate Bacillus siamese B-3 three times on beef juice solid medium, then pick a single colony and inoculate it in a 250mL Erlenmeyer flask containing 50mL beef juice liquid medium, at a constant temperature of 30°C and 200r / min Cultivate on a shaker for 2 days to obtain a culture solution.

[0085] 2. After completing step 1, take the culture medium in step 1, centrifuge at 12000r / min for 1min, discard the supernatant, and collect the precipitate.

[0086] 3. After completing step 2, take the precipitate in step 2 and resuspend it with 50mL sterile water to obtain a bacterial suspension (the concentration of Bacillus siamese B-3 is about 1.0×10 11 cfu / mL).

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Abstract

The invention discloses Bacillus siamensis B-3 and an application thereof. The collection number of the Bacillus siamensis B-3 at China General Microbiological Culture Collection Center is CGMCC No. 13753. Experiments prove that a strain obtained by culturing the Bacillus siamensis B-3 can inhibit growth of Streptomyces scabies, Streptomyces acidiscabies and / or Streptomyces albidoflavus causing potato scab, and the Bacillus siamensis B-3 and the related strain can be used for preventing and treating the potato scab.

Description

technical field [0001] The invention relates to the bacillus siamese B-3 and its application in the field of biotechnology. Background technique [0002] Potato scab is a typical soil-borne disease, which occurs in every potato producing area in my country. The main manifestation is the appearance of nearly round to amorphous suberized scab-like light brown lesions or plaques on the surface of potato tubers, and the texture is rough to the touch. Potato scab generally has two symptoms of reticulated lesions and cracked lesions. Usually, reticulated lesions and cracked lesions are limited to the cortex. When the disease is severe, the lesions are contiguous and cannot be stored, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, which greatly reduces the The quality and economic value, and the diseased seed potatoes can be transported to accelerate the spread of the disease. It poses a serious threat to my country's potato p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 张国青仲乃琴钞亚鹏赵盼杨敬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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