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Streptomyces flavotricini for antagonizing botryosphaeria dothidea

A technology of Streptomyces flavitinus and Streptomyces flavitinus, applied in the field of biological control of plant diseases, can solve the problems affecting the yield and quality of bayberry, insufficient disease control, and weak tree vigor

Inactive Publication Date: 2014-06-18
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Red bayberry canker is an important disease in many bayberry planting areas, which can easily cause tree vigor to weaken and affect the yield and quality of bayberry
At present, the method of preventing and controlling the disease is mainly to use the chemical agents Yebuzol and agricultural streptomycin to smear, and the control effects of other agents are not ideal.
From the perspective of disease control, only two pesticides are not enough for disease control, and other effective pesticides need to be developed for agricultural production.

Method used

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  • Streptomyces flavotricini for antagonizing botryosphaeria dothidea
  • Streptomyces flavotricini for antagonizing botryosphaeria dothidea
  • Streptomyces flavotricini for antagonizing botryosphaeria dothidea

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Embodiment Construction

[0010] (1) Collect soil samples, remove large particles such as stones, crush and air-dry, and sieve.

[0011] (2) Place 20g of the sample in 200mL of sterile water, shake it fully, and use this solution as the mother solution for gradient dilution.

[0012] (3) Take 10 -6 0.1mL of the diluent was used to coat the No. 1 plate of Gao Shi.

[0013] (4) Culture at 28°C for 7 days.

[0014] (5) Separate and save single colonies of different colors and traits.

[0015] (6) Mix Myrica rubra (the strain was isolated from the diseased strains in Cixi Yangmeiyuan in our laboratory and identified as Myrica rubra by morphology and molecular biology) with the bacterial culture medium at 30°C, and make a plate after cooling. Make a hole on the plate, dig out the agar block, drop 20 μL of the bacterial suspension of the isolated strain into the hole, incubate at 28°C, and observe the size of the inhibition zone. (7) Purify the strains with the inhibition zone for single colony.

[0016...

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Abstract

The invention relates to biological prevention of plant diseases and belongs to the field of biological prevention research and application in a plant protection subject. The invention discloses streptomyces flavotricini YZF3 for antagonizing botryosphaeria dothidea, with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7529. The strain can effectively inhibit the botryosphaeria dothidea and the effect is obviously better than that of bismerthiazol and is equivalent to agricultural streptomycin; and the strain has the potential of preventing and treating the botryosphaeria dothidea. Compared with a chemical medicament, the YZF3 strain is used for preventing and treating the plant diseases and has no problems of environmental pollution, pesticide residues and the like.

Description

technical field [0001] The invention relates to biological control of plant diseases and belongs to the field of biological control research and application in the discipline of plant protection. Background technique [0002] Red bayberry canker is an important disease in many bayberry planting areas, which can easily cause tree vigor to weaken and affect the yield and quality of bayberry. At present, the method of preventing and controlling the disease is mainly to use the chemical agents Yebuzol and agricultural streptomycin to smear, and the control effects of other agents are not ideal. From the perspective of disease control, only two pesticides are not enough for disease control, and other effective pesticides need to be developed for agricultural production. In order to provide a basis for the development of new biopesticides and enrich the types of agents for the prevention and treatment of bayberry canker, an actinomycete YZF3 strain was screened from soil in the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/465
Inventor 童蕴慧陈孝仁郑金土张同心
Owner YANGZHOU UNIV
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