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Streptomyces flavotricini resistant to fish pathogens and application thereof

A technology of Streptomyces aflatoxin and pathogenic bacteria, applied in the direction of antibacterial drugs, bacteria, climate change adaptation, etc., to achieve good antibacterial effect and important application value

Active Publication Date: 2019-08-06
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report about the growth of Streptomyces flavitovii, as fish antagonistic bacteria, inhibiting the growth of fish pathogenic bacteria Aeromonas victorii, Aeromonas hydrophila, Aeromonas caviae and Erwinia

Method used

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  • Streptomyces flavotricini resistant to fish pathogens and application thereof
  • Streptomyces flavotricini resistant to fish pathogens and application thereof
  • Streptomyces flavotricini resistant to fish pathogens and application thereof

Examples

Experimental program
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Embodiment

[0031] ① Streptomyces flavitinus X101 (abbreviated as S.f.X101) of this embodiment was preserved on January 2, 2019 at the China Center for Type Culture Collection (CCTCC, address: Wuhan University, Wuhan, China), and the strain preservation number is CCTCC NO: M 2019004.

[0032] ② The isolation process of Streptomyces flavitinus X101 strain in this example: collect soil samples from the vegetable field at the former site of Hunan Xinmin Society, add sterile water, mix well, and gradiently dilute, take 100 microliters (μL) and evenly spread it on Gaoshi No. 1 On the culture medium plate, pick a single colony from the plate, dilute it again and streak it on the Gaoshi No. 1 medium plate, culture it at 30°C for 72 hours, and inoculate the single colony that was repeatedly isolated and purified into the Gaoshi No. 1 liquid medium. In the bottle, 30°C, 180-200rpm, cultured for 72h, mixed with 50% glycerol, stored in -80°C refrigerator for a long time.

[0033] ③ Optical microsco...

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Abstract

The invention relates to streptomyces flavotricini resistant to fish pathogens and an application thereof, the streptomyces flavotricini of the present invention is streptomyces flavotricini X101, Thestrain is deposited at the China Center for Type Culture Collection on January 2, 2019, and the strain is deposited as CCTCC NO: M 2019004. The invention also includes the application of streptomycesflavotricini, and the fermentation bacteria liquid of streptomyces flavotricini has antibacterial activity against fish pathogens such as Aeromonas veronii, Aeromonas hydrophila, Aeromonas caviae, and Erwinia.

Description

technical field [0001] The present invention relates to a strain of Streptomyces flavotricini X101 resistant to fish pathogenic bacteria and its application, a screened strain of Aeromonas victoria, Aeromonas hydrophila, Aeromonas caviae, Erwinia etc. Biological assays were carried out on the fermentation broth of Streptomyces aflavitinus which had inhibitory effects on the growth of fish pathogens. Background technique [0002] Most Streptomyces have antagonistic effects. More than 60 years of research have shown that Actinomycetes can produce many important active natural products that fight cancer, fight infection and inhibit the growth of other harmful microorganisms. Actinomycetes produce approximately 40% of the approximately 33,500 known bioactive metabolites from bacteria, of which more than 10,400 are from Streptomyces (see Bérdy, János. Thoughts and facts about antibiotics: Where weare now and where we are heading [J]. Journal of Antibiotics, 2012, 65(8): 441.). ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61P31/04C12R1/465
CPCC12N1/20A61P31/04C12R2001/465C12N1/205Y02A40/81
Inventor 丁学知黄海燕夏立秋
Owner HUNAN NORMAL UNIVERSITY
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