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Bacillus atrophaeus and application thereof

A technology of Bacillus atrophs and bacterial agent, applied in the field of microorganisms, can solve problems such as endangering human and animal health, environmental pollution, and drug resistance of pathogenic bacteria

Active Publication Date: 2020-09-15
NINGXIA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the control methods of potato scab mainly include the following types: (1) choose disease-free seed potatoes; (2) apply more organic fertilizer or green manure, which can partially Inhibit the disease; (3) Carry out crop rotation with Cucurbitaceae, Leguminosae, and Liliaceae vegetables for more than 5 years; (4) Choose a vegetable field with good water retention for planting, and water in time in case of drought during the tuber formation period; (5) Chemoprevention: some Chemical pesticides can be used to prevent and treat potato scab, but long-term use of chemical agents can easily cause pathogenic bacteria to develop drug resistance, thereby greatly reducing the control effect, and on the other hand, easily cause serious environmental pollution and endanger the health of humans and animals; (6) Biological control: Screen the microorganisms that have good inhibitory effect on the pathogenic bacteria of potato scab and have strong colonization ability, and apply them in the process of seed potato production and field planting to achieve the effect of inhibiting the growth of pathogenic bacteria

Method used

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  • Bacillus atrophaeus and application thereof
  • Bacillus atrophaeus and application thereof
  • Bacillus atrophaeus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1. Isolation, Identification and Preservation of Bacillus atrophaeus WS-1CGMCC No.16198

[0071] 1. Separation

[0072] 1. Processing of soil samples

[0073] (1) Add 10 g of soil sample (collected from the potato soil in Hailar District, Hulunbeier City, Inner Mongolia Autonomous Region) and an appropriate amount of glass beads to 90 mL of sterile water, shake for 10 min, and let stand for 30 s to make a soil stock solution (at this time, the dilution is recorded as 10 -1 ). Take 1 mL of the supernatant of the soil stock solution and add it to a test tube filled with 9 mL of sterile water and mix well (the dilution is 10 at this time). -2 ), then draw 1mL from this test tube and add it to another test tube filled with 9mL sterile water, mix well, and make a dilution by analogy (the dilution is 10 -4 ).

[0074] 2. Take Streptomyces scab 4.0076, inoculate it on a potato solid plate, and culture it statically at 28°C for 4-5 days; then add 0.2mL sterile water...

Embodiment 2

[0087] Embodiment 2, the propagation of Bacillus atrophaeus WS-1

[0088] The specific steps for the propagation of Bacillus atrophaeus WS-1 are as follows:

[0089] 1. Activate Bacillus atrophaeus WS-1 three times on potato solid medium. Take 1 inoculation loop of Bacillus atrophaeus WS-1, inoculate it into a 250mL Erlenmeyer flask filled with 50mL potato liquid medium, and culture it with shaking at 28°C and 200r / min for 2 days to obtain a bacterial solution.

[0090] 2. After completing step 1, take the bacterial liquid, centrifuge at 12000r / min for 1min, and collect the supernatant and precipitate.

[0091] 3. After completing step 2, take the precipitate and resuspend it with 50 mL of sterile water to obtain a cell suspension (in the cell suspension, the concentration of Bacillus atrophaeus WS-1 is about 1.0×10 10 cfu / mL).

Embodiment 3

[0092] The antibacterial effect of embodiment 3, bacillus atrophaeus WS-1

[0093] 1. The inhibitory effect of Bacillus atrophaeus WS-1 on Streptomyces scab 4.0076

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Abstract

The invention discloses bacillus atrophaeus and an application thereof. The bacillus atrophaeus provided by the invention is bacillus atrophaeus WS-1, and the preservation number of the bacillus atrophaeus WS-1 in the China General Microbiological Culture Collection Center is CGMCC No. 16198. Experiments prove that the bacillus atrophaeus WS-1 can inhibit streptomyces scabies and / or streptomyces acidiscabies and / or streptomyces albidoflavus, and has an important application value for preventing and treating potato scab. The invention has an important application value.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Bacillus atrophaeus and its application. Background technique [0002] Potato scab is a plant disease that harms potato tubers. It is mainly manifested by the appearance of nearly round to amorphous suberized scab-like light brown lesions or plaques on the surface of tubers, and the texture is rough to the touch. Potato scab generally has two symptoms of reticulated lesions and cracked lesions. Usually, reticulated lesions and cracked lesions are limited to the cortex, but the quality and yield of the damaged potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, resulting in certain economic losses. loss. In the past, due to the low incidence rate and small damage area in our country, it did not attract people's attention. However, in re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 熊悯梓仲乃琴钞亚鹏杨敬赵盼刘宁宋双伟郝辽洋
Owner NINGXIA UNIVERSITY
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