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A strain of Streptomyces albidoflavus Z9, and applications of Streptomyces albidoflavus Z9 in prevention and control of sunflower sclerotium blight

A technology of Streptomyces albicans and sunflower, applied in the field of microorganisms, can solve the problems of limited sclerotia effect, tall sunflower plants, and difficulties in field application

Active Publication Date: 2017-12-19
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control mainly uses carbendazim, sclerotin, and sclerotin, but the sunflower plants are too tall, and it is very difficult to apply them in the field. The effect is not ideal
In terms of agricultural control, there is also a lack of economical, practical and effective methods

Method used

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  • A strain of Streptomyces albidoflavus Z9, and applications of Streptomyces albidoflavus Z9 in prevention and control of sunflower sclerotium blight
  • A strain of Streptomyces albidoflavus Z9, and applications of Streptomyces albidoflavus Z9 in prevention and control of sunflower sclerotium blight
  • A strain of Streptomyces albidoflavus Z9, and applications of Streptomyces albidoflavus Z9 in prevention and control of sunflower sclerotium blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening and identification of embodiment one sclerotinia

[0025] The sclerotinia strain of the present invention is isolated from diseased sunflower stalks in Inner Mongolia field. The diseased sunflower stalks picked back from Inner Mongolia were cut open with a knife, and the sclerotia sclerotia were taken out, disinfected with 75% ethanol, rinsed with sterile water for 2-3 times, and placed in potato dextrose agar medium on Cultivate at a constant temperature of 25°C for 3-5 days until the mycelium grows, and then pick the mycelium to a new potato dextrose agar medium for growth (see figure 1 ), the purified Sclerotinia sclerotiorum (Lib.) de Bary was determined to be Sclerotinia sclerotiorum (Lib.) de Bary by sequencing the sequence of the ITS region.

Embodiment 2

[0026] Primary screening and identification of embodiment two bacterial strain Z9

[0027] The method that the present invention screens and prevents the sunflower sclerotinia bacterial strain is as follows:

[0028] 1. Collection of soil samples

[0029] Five soil samples were randomly collected from Qilian County, Qinghai. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.

[0030] 2. Purification and cultivation of isolated strains

[0031] Isolate bacterial strains by dilution coating plate method, weigh 1g soil sample, mix in 9mL sterile water as 10 -1 Diluent, for gradient dilution; use 10 -2 、10 -3 and 10 -4 The sample dilution was coated on each separation medium plate, and each dilution was coated on 2 plates, and the amount of coating on each plate was 0.2 mL. The surface of the agar plate must be dry so that the inoculum can be absorbed immediately; separate t...

Embodiment 3

[0055] Rescreening and Preliminary Application of Example Three Bacterial Strain Z9

[0056] 1. Sunflower seed husk experiment

[0057] The strain Z9 was inoculated in the seed medium (soluble starch 2g, casein hydrolyzate 0.3g, glucose 0.5g, Bacto-yeast extract 0.2g, Bacto-malt extract 0.5g, Calcium carbonate 0.3g, sterile water 100ml, pH 7.2), placed in a full-temperature shaking incubator at 170rpm / min, 28°C constant temperature activation and constant temperature cultivation for 24h. According to the inoculum size of 5% (that is, the seed medium of 10ml is added in the corresponding medium of 190ml), the bacterial strain Z9 in the seed medium is inoculated to No. agar), and then placed in a full-temperature shaking incubator for cultivation, maintaining a rotation speed of 170 rpm / min, controlling the temperature at 28° C., and culturing for 3-5 days.

[0058] Treatment of strain Z9 fermentation liquid: Take out the strain Z9 fermentation liquid in a constant temperature...

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Abstract

The invention discloses a strain of Streptomyces albidoflavus Z9 capable of preventing and controlling sunflower sclerotium blight at a seedling stage, and belongs to the technical field of microorganisms, wherein the strain has the preservation number of CGMCC 13115. According to the present invention, the results of the separation activation and identification of the pathogenic bacteria Sclerotinia scleroterum of sunflower sclerotium blight, the primary screening and secondary screening of antagonistic bacteria, the strain identification analysis of the antagonistic bacteria and other experiments show that the strain Z9 can strongly inhibit the hypha growth of Sclerotinia scleroterum; the peel infection incidence rate of the sunflower seed treated with the strain Z9 fermentation raw liquid is reduced by 70%, the seed germination promotion rate of the strain Z9 fermentation filtrate is 33.3%, the prevention and control effect of the strain Z9 fermentation raw liquid on the ex vivo leaf is 69.6%, the prevention and control effect of the strain Z9 fermentation raw liquid in potted plant experiments is 66.7%, and the strain Z9 can effectively inhibit the growth and reproduction of Sclerotinia scleroterum; and the strain has advantages of simple culture conditions, easy preservation, environment protection, and good development and application value.

Description

technical field [0001] The invention relates to a strain of Streptomyces albidoflavus Z9 and its application in the prevention and treatment of sunflower sclerotinia, more precisely relates to the application of the strain in the prevention and treatment of sclerotinia in the growth process of sunflower seedlings, and belongs to the technical field of microorganisms . Background technique [0002] Sclerotinia sclerotiorum is one of the most important diseases of sunflowers, which occurs in all countries in the world. The pathogen Sclerotinia sclerotiorum is highly pathogenic and can penetrate the epidermis of host plants, kill cells, and then infect sunflowers. Many parts such as roots, stems, leaves and flower discs may even cause the whole or part of the plant to rot and die. If it is not managed properly, it will cause great losses to the sunflower yield. In recent years, the widespread occurrence and serious damage of sunflower sclerotinia have greatly affected the stea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 张建丽郑晓薇庄俊丽单双权郭靖楠
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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