Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Streptomyces cinnamon zx6 for controlling sunflower sclerotinia and its application

A technology of Streptomyces cinnamonis and sunflower, applied in the field of microorganisms, can solve the problems of lack of economy and practicality, limited sclerotium effect, difficulty in field medication, etc.

Active Publication Date: 2020-09-04
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control mainly uses carbendazim, sclerotin, and sclerotinia, but the sunflower plants are too tall, and it is very difficult to apply them in the field. The effect of the chemicals on sclerotia in the soil is limited, so the control effect is not ideal
In terms of agricultural control, there is also a lack of economical, practical and effective methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Streptomyces cinnamon zx6 for controlling sunflower sclerotinia and its application
  • A strain of Streptomyces cinnamon zx6 for controlling sunflower sclerotinia and its application
  • A strain of Streptomyces cinnamon zx6 for controlling sunflower sclerotinia and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening and identification of embodiment one sclerotinia

[0025] The bacterial strain of the sclerotinia of the present invention is isolated from the sunflower stalks in Inner Mongolia. Cut open the sunflower stalks picked back from Inner Mongolia with a knife, take out the Sclerotinia sclerotia sclerotia, disinfect with 75% ethanol, rinse 2-3 times with sterile water, place in potato dextrose agar medium at 25°C Cultivate at constant temperature for 3-5d until the mycelia grow out, then pick the mycelium into a new potato dextrose agar medium for growth, and carry out ITS region sequence sequencing of the purified Sclerotinia sclerotiorum to determine that it is Sclerotinia sclerotiorum (Sclerotinia sclerotiorum ( Lib.) de Bary).

Embodiment 2

[0026] Primary Screening and Identification of Example 2 Bacterial Strain ZX6

[0027] The method that the present invention screens and prevents the sunflower sclerotinia bacterial strain is as follows:

[0028] 1. Collection of soil samples

[0029] Five soil samples were randomly collected from each of Inner Mongolia, Qinghai and Jiuquan. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.

[0030] 2. Purification and cultivation of isolated strains

[0031] Isolate bacterial strains by dilution coating plate method, weigh 1g soil sample, mix in 9mL sterile water as 10 -1 Diluent, for gradient dilution; use 10 -2 、10 -3 and 10 -4 The sample dilution was coated on each separation medium plate, and each dilution was coated on 2 plates, and the amount of coating on each plate was 0.2 mL. The surface of the agar plate must be dry so that the inoculum can be absorbed immedi...

Embodiment 3

[0055] Rescreening and preliminary application of embodiment three bacterial strain ZX6

[0056] 1. Sunflower seed husk experiment

[0057] The strain ZX6 was inoculated in the seed medium (soluble starch 2g, casein hydrolyzate 0.3g, glucose 0.5g, Bacto-yeast extract 0.2g, Bacto-malt extract 0.5g, Calcium carbonate 0.3g, sterile water 100ml, pH7.2), placed in a full-temperature shaking incubator at 170rpm / min, 29°C constant temperature for 3-5d. According to the inoculum size of 5% (that is to say, the seed medium of 10ml is added in the corresponding medium of 190ml), the bacterial strain ZX6 in the seed medium is inoculated to No. agar), and then placed in a full-temperature shaking incubator for cultivation, keeping the rotation speed at 170 rpm / min, controlling the temperature at 29°C, and culturing for 3-5 days.

[0058] Treatment of strain ZX6 fermentation broth: take out the strain ZX6 fermentation broth put into a constant temperature shaking incubator, add it to a 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a strain of Streptomyces cinnamonus ZX6 for preventing and treating sclerotinia in sunflower seedling stage. The preservation number of the strain is CGMCC 13116, which belongs to the technical field of microorganisms. Through the implementation of experiments such as the isolation, activation and identification of the pathogenic sclerotinia of sunflower sclerotinia, the primary screening and re-screening experiments of antagonistic bacteria, and the identification and analysis of antagonistic bacteria, it was shown that the strain ZX6 has a strong ability to inhibit Sclerotinia hyphae ability to grow. The incidence of infection of sunflower seed husks treated by the strain ZX6 fermentation original bacterial liquid of the present invention is reduced to 40%, the promotion rate of bacterial strain ZX6 fermentation filtrate to seed germination is 51.1%, and the isolated leaf control effect of the bacterial strain ZX6 fermentation original bacterial liquid 54%, and the control effect of strain ZX6 fermented original bacterial liquid pot experiment was 58.3%, which can effectively inhibit the growth and reproduction of Sclerotinia sclerotiorum. The bacterial strain of the invention has simple culture conditions, is easy to preserve, is environmentally friendly, and has good development and application value.

Description

technical field [0001] The invention relates to the screening, identification and application of a strain of Streptomyces cinnamonensis ZX6 for preventing and treating sunflower sclerotinia, more precisely, relates to the preliminary application of the strain in the prevention and treatment of sunflower sclerotinia, and belongs to the technical field of microorganisms. Background technique [0002] Sclerotinia sclerotiorum is one of the most important diseases of sunflowers, which occurs in all countries in the world. The pathogen Sclerotinia sclerotiorum is highly pathogenic and can penetrate the epidermis of host plants, kill cells, and then infect sunflowers. Roots, stems, leaves, flower discs and other parts may even cause the whole or part of the plant to rot and die. If not managed properly, it will cause great losses to sunflowers. In recent years, the widespread occurrence and serious damage of sunflower sclerotinia have greatly affected the steady development of sun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 张建丽郑晓薇单双权郭靖楠庄俊丽
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com