Cinnamon streptomyces rubiginosus ZX6 for preventing and treating sunflower sclerotinia rot and application of cinnamon streptomyces rubiginosus ZX6
A technology of Streptomyces cinnamonis and sunflower, applied in the field of microorganisms, can solve the problems of limited sclerotia effect, tall sunflower plants, difficult field medication and the like
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Embodiment 1
[0024] Screening and identification of embodiment one sclerotinia
[0025] The bacterial strain of the sclerotinia of the present invention is isolated from the sunflower stalks in Inner Mongolia. Cut open the sunflower stalks picked back from Inner Mongolia with a knife, take out the Sclerotinia sclerotia sclerotia, disinfect with 75% ethanol, rinse 2-3 times with sterile water, place in potato dextrose agar medium at 25°C Cultivate at constant temperature for 3-5d until the mycelia grow out, then pick the mycelium into a new potato dextrose agar medium for growth, and carry out ITS region sequence sequencing of the purified Sclerotinia sclerotiorum to determine that it is Sclerotinia sclerotiorum (Sclerotinia sclerotiorum ( Lib.) de Bary).
Embodiment 2
[0026] Primary Screening and Identification of Example 2 Bacterial Strain ZX6
[0027] The method that the present invention screens and prevents the sunflower sclerotinia bacterial strain is as follows:
[0028] 1. Collection of soil samples
[0029] Five soil samples were randomly collected from each of Inner Mongolia, Qinghai and Jiuquan. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.
[0030] 2. Purification and cultivation of isolated strains
[0031] Isolate bacterial strains by dilution coating plate method, weigh 1g soil sample, mix in 9mL sterile water as 10 -1 Diluent, for gradient dilution; use 10 -2 、10 -3 and 10 -4 The sample dilution was coated on each separation medium plate, and each dilution was coated on 2 plates, and the amount of coating on each plate was 0.2 mL. The surface of the agar plate must be dry so that the inoculum can be absorbed immedi...
Embodiment 3
[0055] Rescreening and preliminary application of embodiment three bacterial strain ZX6
[0056] 1. Sunflower seed husk experiment
[0057] The strain ZX6 was inoculated in the seed medium (soluble starch 2g, casein hydrolyzate 0.3g, glucose 0.5g, Bacto-yeast extract 0.2g, Bacto-malt extract 0.5g, Calcium carbonate 0.3g, sterile water 100ml, pH7.2), placed in a full-temperature shaking incubator at 170rpm / min, 29°C constant temperature for 3-5d. According to the inoculum size of 5% (that is to say, the seed medium of 10ml is added in the corresponding medium of 190ml), the bacterial strain ZX6 in the seed medium is inoculated to No. agar), and then placed in a full-temperature shaking incubator for cultivation, keeping the rotation speed at 170 rpm / min, controlling the temperature at 29°C, and culturing for 3-5 days.
[0058] Treatment of strain ZX6 fermentation liquid: Take out the strain ZX6 fermentation liquid in a constant temperature shaking incubator, add it to a 50ml ...
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