A strain of Streptomyces albicans z9 and its application in the control of sunflower sclerotinia

A technology of Streptomyces japonica and sunflower, applied in the field of microorganisms, can solve the problems of lack of economy and practicality, limited sclerotium effect, difficulty in field medication, etc.

Active Publication Date: 2020-07-10
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control mainly uses carbendazim, sclerotin, and sclerotin, but the sunflower plants are too tall, and it is very difficult to apply them in the field. The effect is not ideal
In terms of agricultural control, there is also a lack of economical, practical and effective methods

Method used

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  • A strain of Streptomyces albicans z9 and its application in the control of sunflower sclerotinia
  • A strain of Streptomyces albicans z9 and its application in the control of sunflower sclerotinia
  • A strain of Streptomyces albicans z9 and its application in the control of sunflower sclerotinia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening and identification of embodiment one sclerotinia

[0025] The sclerotinia strain of the present invention is isolated from diseased sunflower stalks in Inner Mongolia field. The diseased sunflower stalks picked back from Inner Mongolia were cut open with a knife, and the sclerotia sclerotia were taken out, disinfected with 75% ethanol, rinsed with sterile water for 2-3 times, and placed in potato dextrose agar medium on Cultivate at a constant temperature of 25°C for 3-5 days until the mycelium grows, and then pick the mycelium to a new potato dextrose agar medium for growth (see figure 1 ), the purified Sclerotinia sclerotiorum (Lib.) de Bary was determined to be Sclerotinia sclerotiorum (Lib.) de Bary by sequencing the sequence of the ITS region.

Embodiment 2

[0026] Primary screening and identification of embodiment two bacterial strain Z9

[0027] The method that the present invention screens and prevents the sunflower sclerotinia bacterial strain is as follows:

[0028] 1. Collection of soil samples

[0029] Five soil samples were randomly collected from Qilian County, Qinghai. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.

[0030] 2. Purification and cultivation of isolated strains

[0031] Isolate bacterial strains by dilution coating plate method, weigh 1g soil sample, mix in 9mL sterile water as 10 -1 Diluent, for gradient dilution; use 10 -2 、10 -3 and 10 -4 The sample dilution was coated on each separation medium plate, and each dilution was coated on 2 plates, and the amount of coating on each plate was 0.2 mL. The surface of the agar plate must be dry so that the inoculum can be absorbed immediately; separate t...

Embodiment 3

[0055] Rescreening and Preliminary Application of Example Three Bacterial Strain Z9

[0056] 1. Sunflower seed husk experiment

[0057] The strain Z9 was inoculated in the seed medium (soluble starch 2g, casein hydrolyzate 0.3g, glucose 0.5g, Bacto-yeast extract 0.2g, Bacto-malt extract 0.5g, Calcium carbonate 0.3g, sterile water 100ml, pH 7.2), placed in a full-temperature shaking incubator at 170rpm / min, 28°C constant temperature activation and constant temperature cultivation for 24h. According to the inoculum size of 5% (that is, the seed medium of 10ml is added in the corresponding medium of 190ml), the bacterial strain Z9 in the seed medium is inoculated to No. agar), and then placed in a full-temperature shaking incubator for cultivation, maintaining a rotation speed of 170 rpm / min, controlling the temperature at 28° C., and culturing for 3-5 days.

[0058] Treatment of strain Z9 fermentation liquid: Take out the strain Z9 fermentation liquid in a constant temperature...

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Abstract

The invention discloses a strain of Streptomyces albus Z9 for preventing and treating sclerotinia in sunflower seedling stage. The preservation number of the strain is CGMCC 13115, which belongs to the technical field of microorganisms. Through the implementation of experiments such as the isolation, activation and identification of the pathogenic sclerotinia of sunflower sclerotinia, the primary screening and re-screening experiments of antagonistic bacteria, and the identification and analysis of antagonistic bacteria, it was shown that the strain Z9 has a strong ability to inhibit the hyphae of Sclerotinia sclerotiorum ability to grow. The infection rate of sunflower seed husks treated by the strain Z9 fermented original bacterial liquid of the present invention is reduced by 70%, the germination promotion rate of the bacterial strain Z9 fermentation filtrate is 33.3%, and the control effect of the isolated leaves of the bacterial strain Z9 fermented original bacterial liquid 69.6%, and the pot experiment control effect of bacterial strain Z9 fermented original bacterium solution is 66.7%, which can effectively inhibit the growth and reproduction of Sclerotinia sclerotiorum. The bacterial strain of the invention has simple culture conditions, is easy to preserve, is environmentally friendly, and has good development and application value.

Description

technical field [0001] The invention relates to a strain of Streptomyces albidoflavus Z9 and its application in the prevention and treatment of sunflower sclerotinia, more precisely relates to the application of the strain in the prevention and treatment of sclerotinia in the growth process of sunflower seedlings, and belongs to the technical field of microorganisms . Background technique [0002] Sclerotinia sclerotiorum is one of the most important diseases of sunflowers, which occurs in all countries in the world. The pathogen Sclerotinia sclerotiorum is highly pathogenic and can penetrate the epidermis of host plants, kill cells, and then infect sunflowers. Many parts such as roots, stems, leaves and flower discs may even cause the whole or part of the plant to rot and die. If it is not managed properly, it will cause great losses to the sunflower yield. In recent years, the widespread occurrence and serious damage of sunflower sclerotinia have greatly affected the stea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P21/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 张建丽郑晓薇庄俊丽单双权郭靖楠
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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