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Preparation method and application of bisphenol A electrochemical luminescence aptamer sensor

An aptamer sensor, electrochemical technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc., to achieve the effect of improving stability, high sensitivity, and solving poor stability

Inactive Publication Date: 2019-05-07
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, without any specific recognition element, the selectivity of this system needs to be further improved

Method used

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  • Preparation method and application of bisphenol A electrochemical luminescence aptamer sensor
  • Preparation method and application of bisphenol A electrochemical luminescence aptamer sensor
  • Preparation method and application of bisphenol A electrochemical luminescence aptamer sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] according to figure 1 Described preparation process:

[0032](1) Preparation of NCDs@PEI-reduced graphene oxide (rGO) composites

[0033] First, add 3g of citric acid, 3g of urea and 6g of polyethyleneimine (PEI) into 10mL of secondary water, react in an 800W microwave oven for 5min, and after cooling to room temperature, the obtained product is dissolved in secondary water again, at 12000rpm Centrifuge for 20min. The resulting upper layer solution was dialyzed in secondary water with a 50 kDa dialysis bag for three days and three nights to remove unreacted precursors. Finally, dry and concentrate in a vacuum oven at 60°C.

[0034] NCDs@PEI (2mg·mL) obtained above -1 ) was added to an equal volume of 2mg·mL -1 In rGO, mix ultrasound to prepare uniform NCDs@PEI-rGO (1mg mL -1 ), placed at room temperature for later use.

[0035] (2) The glassy carbon electrode (d=3mm GCE) was ground successively with 0.3 μm and 0.05 μm Al2O3 powder, and dried in air after ultrason...

Embodiment 2

[0045] (1) Preparation of NCDs@PEI-reduced graphene oxide (rGO) composites

[0046] First, add 3g of citric acid, 3g of urea and 6g of polyethyleneimine (PEI) into 10mL of secondary water, react in an 800W microwave oven for 5min, and after cooling to room temperature, the obtained product is dissolved in secondary water again, at 12000rpm Centrifuge for 20min. The resulting upper layer solution was dialyzed in secondary water with a 50 kDa dialysis bag for three days and three nights to remove unreacted precursors. Finally, dry and concentrate in a vacuum oven at 60°C.

[0047] NCDs@PEI (2mg·mL) obtained above -1 ) was added to an equal volume of 2mg·mL -1 In rGO, mixed with ultrasound to prepare uniform NCDs@PEI-rGO (1mg·mL -1 ), placed at room temperature for later use.

[0048] (2) The glassy carbon electrode (d=3mm GCE) was ground successively with 0.3 μm and 0.05 μm Al2O3 powder, and dried in air after ultrasonication in ethanol and water.

[0049] (3) 3 μL 0.5mmoL...

Embodiment 3

[0057] (1) Preparation of NCDs@PEI-reduced graphene oxide (rGO) composites

[0058] First, add 3g of citric acid, 3g of urea and 6g of polyethyleneimine (PEI) into 10mL of secondary water, react in an 800W microwave oven for 5min, and after cooling to room temperature, the obtained product is dissolved in secondary water again, at 12000rpm Centrifuge for 20min. The resulting upper layer solution was dialyzed in secondary water with a 50 kDa dialysis bag for three days and three nights to remove unreacted precursors. Finally, dry and concentrate in a vacuum oven at 60°C.

[0059] NCDs@PEI (2mg·mL) obtained above -1 ) was added to an equal volume of 2mg·mL -1 In rGO, mixed with ultrasound to prepare uniform NCDs@PEI-rGO (1mg·mL -1 ), keep at room temperature for later use.

[0060] (2) The glassy carbon electrode (d=3mm GCE) was ground successively with 0.3 μm and 0.05 μm Al2O3 powder, and dried in air after ultrasonication in ethanol and water.

[0061] (3) 3 μL 0.5mmoL·L...

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Abstract

The invention belongs to the technical field of biosensing detection, and relates to a preparation method and application of a bisphenol A electrochemical luminescence aptamer sensor. The preparationmethod and the application of the bisphenol A electrochemical luminescence aptamer sensor is specifically a novel electrochemical luminescence (ECL) aptamer sensor based on poly(ethyleneimine) functionalized nitrogen-doped carbon nano-dots (NCDs@PEI)) as a Rucbpy nanosheet (RuNSs) co-reactant for the specific detection of the bisphenol A (BPA). In the invention, due to the dual enhancement of PEIand NCDs on RuNSs, NCDs@PEI can be used as an effective co-reactant for RuNSs, which greatly improves the luminescence efficiency of a system and enhances a luminescent signal. In order to improve theselectivity of a RuNSs-NCDs@PEI system for BPA detection, inventors innovatively introduce a BPA aptamer to construct a novel ECL aptamer sensor. The linear response of a constructed ECL aptamer sensor to BPA is 1.0x10<-10>-1.0x10<-4> mol L<-1>, and the detection limit is 3.3x10-11 mol L<-1>. The electrochemical luminescence aptamer sensor of the invention has good selectivity and high sensitivity and provides a novel aptamer sensing platform for determining the BPA of an actual sample.

Description

technical field [0001] The invention belongs to the technical field of biosensing and detection, and relates to a preparation method and application of a bisphenol A electrochemiluminescent aptamer sensor, in particular to a bisphenol A electrochemiluminescent aptamer based on RuNSs-NCDs@PEI system The preparation method and application of the sensor. Background technique [0002] Bisphenol A (BPA), as an industrial synthetic organic chemical, is widely used in the synthesis of polycarbonate plastics and epoxy resins. However, excess BPA can increase the incidence of cancer, reduce fertility, reduce immune function, and cause other unknown diseases in humans and animals. Therefore, the detection of BPA is necessary. At present, the methods for detecting BPA mainly include high-performance liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), capillary electrophoresis-ultraviolet spectroscopy (CE-UV), and enzyme-linked immunosorben...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N21/76
Inventor 由天艳刘晓红李丽波罗莉君张家一
Owner JIANGSU UNIV
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