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Method for eliminating false positive interference of G test

A technology of false positives and interfering substances, applied in the field of G test detection, can solve the problems of false positive results of test results, delaying diagnosis and treatment, affecting the clinical diagnosis of deep fungal infection, etc., and achieve the effect of accurate detection and elimination of interference effects.

Active Publication Date: 2015-04-01
湛江安度斯生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, some patients, such as 1. Patients who have been injected with fat emulsion; 2. Patients who have been infused intravenously with immunoglobulin, albumin, coagulation factors or blood products; 3. Patients who have taken sulfa drugs have plasma / serum containing Non-specific Limulus reagent reactants, which will lead to a decrease in the light transmittance of the reaction solution during the reaction
The luminosity changes caused by this non-specific LAL reactant will lead to false positive results in the test results, which seriously affects the clinical diagnosis of deep fungal infections, and usually requires patients to re-take blood for testing after stopping the drug, which will delay diagnosis and treatment.

Method used

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  • Method for eliminating false positive interference of G test
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  • Method for eliminating false positive interference of G test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of blood samples to be tested:

[0033] Blood sample 1: normal G test positive human plasma / serum;

[0034] Blood sample 2: Plasma / serum of patients who have been injected with fat emulsion;

[0035] The test materials of this embodiment are as follows: Magnesium sulfate (MgSO 4 ); trishydroxymethylaminomethane (C 4 h 11 NO 3 ); Hydrochloric acid (HCL); Bacterial endotoxin test water (W); The sample to be tested is the test article (S).

[0036] The test method and steps are as follows:

[0037] S1. Take a small amount of pyrogen-free magnesium sulfate (MgSO 4 ) and Tris (Tris, C 4 h 11 NO 3 ), dissolved in water for bacterial endotoxin inspection, and configured to contain 0.3M MgSO 4 and 0.05M Tris buffer, and then adjust the pH value to 6.0;

[0038] S2. Dilute the two human plasma / serum mentioned above 10 times with water for bacterial endotoxin testing, heat at 70°C for 10 minutes, and then cool to room temperature to prepare a 10-fold diluti...

Embodiment 2

[0044] Preparation of blood samples to be tested:

[0045] Blood sample 1: normal G test positive human plasma / serum;

[0046] Blood sample 2: Plasma / serum from patients receiving intravenous immunoglobulin;

[0047] The test materials of this embodiment are as follows: Magnesium sulfate (MgSO 4 ); trishydroxymethylaminomethane (C 4 h11 NO 3 ); Hydrochloric acid (HCL); Bacterial endotoxin test water (W); The sample to be tested is the test article (S).

[0048] The test method and steps are as follows:

[0049] S1. Take a small amount of pyrogen-free magnesium sulfate (MgSO 4 ) and Tris (Tris, C 4 h 11 NO 3 ), dissolved in water for bacterial endotoxin inspection, and configured to contain 0.5M MgSO 4 and 0.15M Tris buffer, then adjust the pH value to 7.0;

[0050] S2. Dilute the above two kinds of human plasma / serum 10 times with water for bacterial endotoxin test, heat at 73°C for 12 minutes, and then cool to room temperature to prepare a 10-fold dilution of human ...

Embodiment 3

[0056] Preparation of blood samples to be tested:

[0057] Blood sample 1: normal G test positive human plasma / serum;

[0058] Blood sample 2: plasma / serum of patients who have taken sulfa drugs;

[0059] The test materials of this embodiment are as follows: Magnesium sulfate (MgSO 4 ); trishydroxymethylaminomethane (C 4 h 11 NO 3 ); Hydrochloric acid (HCL); Bacterial endotoxin test water (W); The sample to be tested is the test article (S).

[0060] The test method and steps are as follows:

[0061] S1. Take a small amount of pyrogen-free magnesium sulfate (MgSO 4 ) and Tris (Tris, C 4 h 11 NO 3 ), dissolved in water for bacterial endotoxin inspection, and configured to contain 0.8M MgSO 4 and 0.2M Tris buffer, then adjust the pH value to 8.0;

[0062] S2. Dilute the above two kinds of human plasma / serum 10 times with water for bacterial endotoxin test, heat at 75°C for 15 minutes, and then cool to room temperature to prepare a 10-fold dilution of human plasma / seru...

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Abstract

The invention relates to the G test detection technical field, and particularly discloses a method for eliminating false positive interference of a G test, that is to say, a buffer solution containing a divalent soluble metal salt and Tris is prepared and is mixed with patient plasma or serum containing an interfering substance to prepare a detection sample, and the detection sample is subjected to the G test. With the use of the method, when the patient plasma / serum containing a nonspecific tachypleus amebocyte lysate is detected, the interference effect can be eliminated, the beta-G concentration in the patient plasma / serum is accurately detected, and patient mental and economic burdens caused by clinical misjudgment are greatly reduced.

Description

technical field [0001] The invention relates to the technical field of G test detection, more specifically, relates to a method for eliminating false positive interference of G test. Background technique [0002] Fungi widely exist in nature, including the human mouth, nasopharynx, intestinal tract, female reproductive tract, etc. However, the clinical manifestations of deep fungal infections caused by these normal flora are extremely atypical, and it is difficult to distinguish them from other bacterial infections. If the fungus is not treated in time, its prognosis is poor. Therefore, we strive to find a correct and rapid way to judge deep fungi clinically. (1,3)-β-D glucan (β-G) is one of the components of some fungal cell walls, such as Candida and Aspergillus. When the bacteria metabolize or die, the β-G component in the cell wall will be released into the environment. Detecting the serum or plasma of patients with Candida or Aspergillus deep infection, it will be fou...

Claims

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Application Information

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IPC IPC(8): G01N33/96
CPCG01N1/28
Inventor 熊向党李树馀
Owner 湛江安度斯生物有限公司
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