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Method for detecting bacterial endotoxin of hemoglobin sample

A technology of bacterial endotoxin and hemoglobin, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems of unable to detect endotoxin in hemoglobin samples, and achieve high sensitivity, The effect of reducing interference and the method is simple and easy to implement

Inactive Publication Date: 2018-12-18
REDPHARM BEIJING BIOPHARMACEUTICAL INST CO LTD +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN102901726 discloses the preparation and application of a blood bacterial endotoxin detection kit. When the kit of the invention is used for blood bacterial endotoxin detection, the blood sample needs to be centrifuged first, and the blood bacteria are eliminated after centrifugation. Red blood cells, thereby eliminating the influence of hemoglobin on the detection of bacterial endotoxin, and the endotoxin detection of hemoglobin samples cannot be performed with this kit

Method used

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  • Method for detecting bacterial endotoxin of hemoglobin sample
  • Method for detecting bacterial endotoxin of hemoglobin sample
  • Method for detecting bacterial endotoxin of hemoglobin sample

Examples

Experimental program
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Embodiment 1

[0028] 1. Prepare endotoxin standard solution with endotoxin test water.

[0029] 1. Take a bottle of endotoxin, remove the metal cap, slowly open the bottle stopper, add 5.0ml of endotoxin inspection water with a 1000ul pipette, cover the bottle stopper, seal it with a sealing film, and mix on a vortex shaker Mix well, mix once every 5min, 1min each time, 60-120min in total. The concentration is 1000EU / ml.

[0030] 2. In a pyrogen-free test tube, add 1800ul of water for endotoxin testing, add 200ul of the sample from step 1, and mix on a vortex shaker for about 1min. The concentration is 100EU / ml.

[0031] 3. In a pyrogen-free test tube, add 1800ul of water for endotoxin testing, add 200ul of the sample from step 2, and mix on a vortex shaker for about 1min. The concentration is 10 EU / ml.

[0032] 4. In a pyrogen-free test tube, add 1800ul of water for endotoxin testing, add 200ul of the sample from step 3, and mix on a vortex shaker for about 1min. The concentration is ...

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Abstract

The invention discloses a method for detecting bacterial endotoxin of a hemoglobin sample. The method comprises the following steps of a, dissolving tachypleus amebocyte lysate dry powder by using a Gfactor blocking agent; b, diluting the hemoglobin sample for 20 to 40 times; and c, fully and uniformly mixing a reactant obtained in the step a and b, incubating at 37 DEG C, monitoring absorbance at 660 nm, and carrying out bacterial endotoxin detection. The G factor blocking agent adopted in the step a adds trasylol and pachymaran in purified water, so that each milliliter of blocking agent solution contains 5 to 15 micrograms of trasylol and 10 to 20 micrograms of pachymaran. The method for detecting the bacterial endotoxin of the hemoglobin sample is low in detection limit, high in accuracy and easy to operate, and is capable of realizing accurate detection of the bacterial endotoxin of the hemoglobin sample.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a bacterial endotoxin detection method of a hemoglobin sample. Background technique [0002] Endotoxin is a general term for toxic substances present in the cells of Gram-negative bacteria, and its main component is phospholipopolysaccharide, which can cause fever, so it is also called a 'pyrogen'. [0003] Endotoxin is a potential pollutant of biological products. For infusion products, a reliable and effective method is needed to quantify endotoxin residues. [0004] The detection methods of endotoxin can be divided into gel method and photometric method. The gel method is a qualitative detection or semi-quantitative detection based on the principle of agglutination reaction between the limulus reagent and endotoxin. Photometric methods include turbidity and chromogenic matrix methods, which are further divided into endpoint turbidimetric methods, dynamic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/82
CPCG01N21/82
Inventor 游可为闫晓玲孙新宇刘迎史国营陈浩源
Owner REDPHARM BEIJING BIOPHARMACEUTICAL INST CO LTD
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