The invention discloses a method for determining the content of platycodon grandiflorum. The method comprises the following steps: filtering platycodon grandiflorum
powder by utilizing a number-two screen, mixing 2g of filtered platycodon grandiflorum
powder with 50ml of 50%
methanol to obtain a mixture, carrying out ultrasonic treatment on the mixture for 30 minutes, cooling and complementing, filtering, and recovering a
solvent until the
solvent is completely recovered; extracting residues, washing the residues,
drying an
organic layer by
distillation, dissolving the
organic layer by utilizing
methanol, separating by utilizing a
silica gel column, eluting and separating for multiple times, collecting an
elution solution,
drying the
elution solution by
distillation, fixing the constant volume, filtering and finally obtaining a sample; and separating, and calculating the content of the platycodon grandiflorum under experimental conditions that a high-efficiency liquid-phase chromatograph adopts a
chromatographic column Agilent TC-C18 5 micrometers, 4.6*150mm, the
column temperature is 30 DEG C, the flow rate is 1.0ml / L, and the ratio of
acetonitrile to water is 25: 75. After multiple tests, the content of the platycodon grandiflorum is subjected to the great influence of different silica gels, the content of the platycodon grandiflorum is very high by virtue of the extraction of the
silica gel produced in Qingdao sea, the platycodon grandiflorum is generally qualified, the problem that the content of the existing platycodon grandiflorum cannot reach the standard can be effectively solved, and the test difficulty of
medicine enterprises utilizing pharmacopeia can be solved.