Method for detecting endotoxin in alcohol solution

A technology of endotoxin and bacterial endotoxin, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of polluting endotoxin, unqualified products, affecting corporate reputation and image, etc., to reduce alcohol content, reduce Influence and improve the effect of the overall detection accuracy

Pending Publication Date: 2020-10-30
斯坦利思生物科技(杭州)有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Every link in biopharmaceuticals may contaminate endotoxin and make the product unqualified, which not only causes great economic losses to the manufacturer, but also directly affects the reputation and image of the company

Method used

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  • Method for detecting endotoxin in alcohol solution

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] A method for detecting endotoxin in alcohol solution, comprising the steps of:

[0032] Step 1. Preparation before testing: Prepare the containers and solutions required for testing, including any of the contents of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. Alcohol solution, more than ten glass containers filled with liquid, constant temperature dry heat box, pipette, experimental water, some 75% alcohol cotton balls, grinding wheel, sealing...

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Abstract

The invention discloses a method for detecting endotoxin in an alcohol solution. The method comprises the following steps: heating an alcohol solution, and carrying out endotoxin detection on the treated alcohol solution by using a tachypleus amebocyte lysate detection method; and through a heating evaporation method, taking out the alcohol in the solution as much as possible on the premise that endotoxin is not reduced. According to the method, the alcohol solution is heated, then the treated alcohol solution is subjected to endotoxin detection by using a tachypleus amebocyte lysate detectionmethod, and on the basis of the support of the prior art, the pertinence of detection is improved in combination with the expansion of a self mode; and alcohol in the solution is taken out as much aspossible by utilizing a heating evaporation method on the premise of not reducing endotoxin, so that the overall alcohol content in the detection solution is reduced, the overall detection accuracy of the device is improved, and the influence of the alcohol content on a detection result is reduced.

Description

technical field [0001] The invention relates to the technical field of endotoxin detection methods in alcoholic solutions, in particular to a method for detecting endotoxins in alcoholic solutions. Background technique [0002] Bacterial endotoxin is a complex of lipopolysaccharide and trace protein on the cell wall of Gram-negative bacteria. Toxin Biologically active substance. The harm of endotoxin is very serious, it will cause: 1. Fever reaction: The human body is extremely sensitive to bacterial endotoxin. A very small amount (1-5ng / kg) of endotoxin can cause body temperature to rise; 2. Leukocyte reaction: After bacterial endotoxin enters the host body, due to the movement of cells and adhesion to tissue capillaries, the blood stream accounts for 60% of the total number of white blood cells ~70% of the neutrophils decrease rapidly; 3. Endotoxin shock: a large amount of endotoxin acts on the body's macrophages, neutrophils, complement system and coagulation system, et...

Claims

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Application Information

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IPC IPC(8): G01N33/579G01N33/68
CPCG01N33/579G01N33/68
Inventor 毛斌杰褚培忠陈杭
Owner 斯坦利思生物科技(杭州)有限公司
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