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Plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and use method thereof

A technology of bacterial endotoxin and diluent, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of complicated operation of turbidity method, expensive equipment, protein influence, etc., achieve low cost, improve work quality and work efficiency, and operate simple effect

Inactive Publication Date: 2018-12-07
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the gel method is easily affected by anticoagulants such as heparin sodium and sodium citrate in the plasma, and the protein in the plasma, the operation of the turbidity method is complicated, and the equipment is expensive. Plasma diluent kit for detection of plasma by bacterial endotoxin gel method and method of use thereof

Method used

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  • Plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and use method thereof
  • Plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and use method thereof
  • Plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and use method thereof

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specific Embodiment approach 1

[0010] Specific embodiment one: In this embodiment, a blood plasma diluent kit for detection of plasma by bacterial endotoxin gel method comprises diluent A, diluent B and diluent C; wherein said diluent A has a concentration of 0.1-0.3mg / mL protamine sulfate solution; Diluent B is CaCl with a concentration of 8.0-10.0mg / mL 2 Solution; dilution C consists of Hepes and Triton X-100, the final concentration of Hepes in dilution C is 8-10mmol / L, and the final concentration of Triton X-100 is 0.3%-0.5%.

[0011] The beneficial effect of this implementation mode:

[0012] This embodiment adopts different reagents for anti-interference dilution according to the different action principles of different anticoagulants, which is highly targeted, effective, and the dilution factor is small, only 8 times of dilution is enough. The whole experiment operation is simple, without adding other auxiliary equipment , no need to change the experimental equipment and equipment, the cost is low, ...

specific Embodiment approach 2

[0013] Embodiment 2: This embodiment differs from Embodiment 1 in that the diluent A is a protamine sulfate solution with a concentration of 0.2 mg / mL. Others are the same as those in Embodiment 1 or 2.

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the diluent B is CaCl with a concentration of 9.0 mg / mL 2 solution. It is the same as the specific embodiment 1 or 2.

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Abstract

The invention relates to a plasma diluent kit for bacterial endotoxin gel process detection of blood plasma and a use method thereof. The invention aims to solve the problems of easy influence of heparin sodium, sodium citrate and other anticoagulants in blood plasma and protein in blood plasma on the gel process, complex operation of the turbidimetric process, and expensive equipment. The kit comprises a diluent A, a diluent B and a diluent C. The kit adopts different diluents for dilution of the blood plasma of different anticoagulants, targetedly eliminates the interference of anticoagulants on tachypleus amebocyte lysate gel reaction, also can eliminate protein in blood plasma, and prevent the interference of protein. Also the dilution ratio is smaller, and a dilution ratio of 8 and conventional tachypleus amebocyte lysate sensitivity can achieve detection. The kit provided by the invention can be applied to the plasma endotoxin detection field.

Description

technical field [0001] The invention relates to a blood plasma diluent kit for detecting blood plasma by a bacterial endotoxin gel method and a use method thereof. Background technique [0002] Biological products containing plasma components, including platelet lysate, platelet-rich plasma, and ordinary plasma, are widely used in orthopedics, dermatology, ophthalmology and other departments. If bacterial endotoxin contaminates such products, it can cause chills and fever, decreased leukocytes, increased vascular permeability and other "pyrogenic reactions" after application. Therefore, the detection of plasma endotoxin content is of great significance in clinical and reinfusion applications. The current method for detecting plasma endotoxins is specified in the Pharmacopoeia as gel method and turbidimetric method. Although the turbidimetric method has the characteristics of high sensitivity, the operation is more complicated and requires the purchase of additional expensive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
Inventor 赵新新郭晶张怡刘艳青刘春香赵晶姜伟娜
Owner 天晴干细胞股份有限公司
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