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Preparation method of double-emission carbon-based nano probe and product of preparation method

A carbon-based nano, dual-emission technology, applied in nanotechnology, nanotechnology, nano-optics and other directions, can solve problems such as uncontrollable, complex process, decreased quantum efficiency, etc., to achieve improved sensitivity, simple preparation method, and product particle size. uniform effect

Active Publication Date: 2018-11-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these processes are complex and uncontrollable, which eventually lead to the decrease of quantum efficiency

Method used

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  • Preparation method of double-emission carbon-based nano probe and product of preparation method
  • Preparation method of double-emission carbon-based nano probe and product of preparation method
  • Preparation method of double-emission carbon-based nano probe and product of preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Weigh 1g of citric acid and 2g of urea and dissolve them in 20mL of N,N-dimethylformamide (DMF), transfer the prepared solution to a reaction kettle, and heat at 200°C for 8h to obtain a black viscous solution; Add 40mL sodium hydroxide solution (50mg / mL) to the mixed solution, then remove the supernatant by centrifugation (16000r / min, 10min), collect the precipitate and dissolve it in dilute hydrochloric acid solution (50mL, 5%), after stirring for 10min, pass Centrifuge (16000r / min, 10min) to remove the supernatant, collect the precipitate, dissolve it in deionized water, centrifuge (16000r / min, 10min) twice to remove the precipitate, collect the supernatant, freeze-dry at -35°C for 72h , to obtain red fluorescent carbon dots. The product was redispersed in water, and its transmission electron microscope picture was as follows figure 1 As shown, the particle size of the product is uniform, 3-5nm, and has good dispersibility.

[0066] Weigh 1 g of citric acid and 0.8...

Embodiment 2

[0070] Weigh 0.6g of p-phenylenediamine and 2g of urea and dissolve them in 20mL of N,N-dimethylformamide (DMF), transfer the prepared solution to a reaction kettle, and heat at 200°C for 8 hours to obtain a black viscous solution; add 40mL potassium hydroxide solution (50mg / mL) to the mixture, then remove the supernatant by centrifugation (16000r / min, 10min), collect the precipitate and dissolve it in dilute nitric acid solution (50mL, 5%), and stir for 10min Finally, remove the supernatant by centrifugation (16000r / min, 10min), collect the precipitate, dissolve it in deionized water, and centrifuge (16000r / min, 10min) twice to remove the precipitate, collect the supernatant, and store at -50°C Freeze-dry for 45 hours to obtain red fluorescent carbon dots.

[0071] Weigh 1 g of glucose and 0.5 g of urea and dissolve them in 10 mL of deionized water, transfer the solution to a reaction kettle, and heat at a constant temperature of 120 °C for 8 h to obtain a blue transparent so...

Embodiment 3

[0074] Weigh 0.5g of o-phenylenediamine and 2g of ethylenediamine and dissolve in 20mL of dimethyl sulfoxide (DMSO), transfer the prepared solution to a reaction kettle, and heat at 200°C for 8 hours to obtain a black viscous solution; Add 40mL sodium hydroxide solution (50mg / mL) to the mixture, then remove the supernatant by centrifugation (16000r / min, 10min), collect the precipitate and dissolve it in dilute hydrochloric acid solution (50mL, 5%), after stirring for 10min, Remove the supernatant by centrifugation (16000r / min, 10min), collect the precipitate, dissolve it in deionized water, centrifuge (16000r / min, 10min) twice to remove the precipitate, collect the supernatant and dry it in vacuum to obtain red fluorescent carbon point.

[0075] Weigh 1 g of fructose and 0.5 g of urea and dissolve in 10 mL of deionized water, transfer the solution to a reaction kettle, and heat at a constant temperature of 120 °C for 8 h to obtain a blue-green transparent solution. The mixed ...

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Abstract

The invention discloses a preparation method of a double-emission carbon-based nano probe prepared based on an electrostatic interaction induced self-assembling method and application of a product ofthe preparation method to in-vivo and in-vitro temperature detection of organisms, and belongs to the technical field of colorimetric fluorescence detection. The preparation method comprises synthesisof blue fluorescence carbon dots (B-CDs) which have no temperature sensitivity and negative charges on surfaces, preparation of red fluorescence carbon dots (R-CDs) which have temperature sensitivityand positive charges on surfaces and preparation of the double-emission carbon-based nano probe. According to the double-emission carbon-based nano probe obtained by the preparation method, the double-emission carbon-based nano probe has different responsiveness aiming at different temperature and has different compound temperature color changes; the double-fluorescence emission nano probe can beused for colorimetrically detecting in-vivo and in-vitro temperature of the organisms by naked eyes, and has high sensitivity (0.93 percent / DEG C) and operation repeatability, so that the double-fluorescence emission nano probe provides convenience for biological imaging, temperature sensing, environment monitoring, food safety and the like.

Description

technical field [0001] The invention specifically relates to a preparation method of a double-emission carbon-based nanomaterial used for temperature sensing and its application in detecting temperature changes in a biological environment, belonging to the technical field of fluorescent nanomaterials. Background technique [0002] Temperature plays a vital role in physiological and biochemical actions and processes in living organisms, and its accurate measurement is of great significance. Therefore, temperature measurement technology has been upgraded from traditional invasive temperature sensors, namely thermocouples and thermistors, to non-invasive Sexy nanometer thermometer. Among them, fluorescent nanothermometers have attracted extensive attention due to their high temporal and spatial resolution and integrated functions of imaging and temperature sensing, and have been successfully applied in biological imaging, temperature sensing, environmental monitoring, or food s...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01K11/16
CPCB82Y20/00B82Y40/00C09K11/65G01K11/16
Inventor 王婵宋启军胡昙昙陈月月
Owner JIANGNAN UNIV
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